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HLA-DRB4 Antibody (MA5-42498) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded human breast carcinoma tissue using HLA-DRB4 Recombinant Monoclonal Antibody (Product # MA5-42498). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the HLA-DRB4... View More
产品信息
MA5-42498
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE55-85
抗原
Synthetic peptide corresponding to the extracellular domain of Human HLA-DRB4
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2911639
产品详细信息
Positive Control: Raji cell lysates, human tonsil tissue, human spleen tissue, human breast carcinoma tissue, Jurkat.
Subcellular Location: Lysosome membrane, Endosome membrane, Late endosome membrane, trans-Golgi network membrane, Endoplasmic reticulum membrane, Cell membrane.
靶标信息
HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DRB1 transplantation antigen; HLA class II histocompatibility antigen, DR beta 4 chain; human leucocyte antigen DRB4; leukocyte antigen; MHC class II antigen DRB4; MHC class II antigen HLA-DR-beta; MHC class2 antigen; MHC HLA DR-beta chain
基因别名: DR-4; DR4; DRB4; HLA-DR4B; HLA-DRB4
UniProt ID: (Human) P13762
Entrez Gene ID: (Human) 3126
