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Application
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HNF4A Antibody (417700) in ICC/IF
Immunofluorescence analysis of HNF4A was performed using 70% confluent log phase HepG2 and HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Anti-HNF4A Monoclonal Antibody (Product # 417700) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjuga... View More
产品信息
417700
应用
建议稀释比
已发表文章
产品规格
已发表种属
Mouse,
Non-human primate
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
H1415
抗原
Baculovirus-expressed recombinant human HNF4-alpha (393-461 aa)
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
保存条件
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
运输条件
Wet ice
RRID
AB_2532197
产品详细信息
This antibody specifically recognizes human HNF4 alpha 1, 2, 4, 5, 7 ,8 and cross reacts with mouse and rat HNF4 alpha 1, 2, 4, 5, 7 ,8.
靶标信息
Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is an orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
