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HPV (Human Papillomavirus), E7 protein Antibody (620-930) in WB
Western Blot: The recombinant protein E7-GST was separated in 10% SDS-PAGE, transferred onto Nitrocellulose membrane, and immunoblotted by Rabbit anti-HPV16/E7(NT)(Cat#620-930) at 1:500. An immunoreactive band is observed at molecular weight ~50 kDa corresponding to the fusion protein size.
产品信息
620-930
应用
建议稀释比
已发表文章
产品规格
种属反应
Virus
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide MHGDTPTLHEYMLDLQPETT corresponding to the N-terminus of human papillomavirus type 16
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with proprietary stabilizer
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Positive control: E7 recombinant protein
靶标信息
The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HPV 16-E7; HPV16E7; human papillomavirus
