Invitrogen

HSC70 Polyclonal Antibody

图: 1 / 1

HSC70 Antibody (PA5-116380) in WB

Western Blot analysis of HSC70 using the HSC70 Polyclonal Antibody (Product # PA5-116380) at a 1:2000 dilution. Lane 1: Zebrafish lysate, Lane 2: Zebrafish muscle lysate, Lane 3: ZF4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 71 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Product Image Gallery

产品信息

PA5-116380

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Zebrafish

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 528-562 amino acids from the C-terminal region of DANRE hspa8

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2901014

靶标信息

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Expression of HSPA14 in patients with acute HIV-1 infection and its effect on HIV-1 replication.

Frontiers in immunology

Bi M,Kang W,Sun Y

PA5-116380 was used in Western Blotting to find that the expression level of HSPA14 is higher in peripheral blood CD4+T cells of untreated acute HIV infection patients with low viral load.