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Invitrogen
HSP27 Monoclonal Antibody (STRSN), eBioscience™
高级验证
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
图: 1 / 10
HSP27 Antibody (14-9112-80) in ICC/IF
Immunofluorescence analysis of HSP27 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HSP27 Monoclonal Antibody (STRSN), eBioscience™ (Product # 14-9112-80) at 2.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ... View More
产品信息
14-9112-80
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
STRSN
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11219272
产品详细信息
Description: The monoclonal antibody STRSN recognizes human heat shock protein 27 (HSP27). The heat shock proteins are a large family of proteins that are induced in response to stressors such as changing temperatures, cytokine levels, and presence of various chemicals. Heat shock proteins serve as molecular chaperones, binding to other proteins to maintain proper protein structure or by folding denatured proteins and preventing activation of caspases that could lead to apoptosis. Unlike the larger HSP proteins, HSP27 belongs to a unique subgroup that is involved in apoptosis regulation. Expression of HSP27 is constitutive, but upon stress HSP27 is upregulated and can relocate from the cytoplasm to the nucleus. Increased HSP27 expression with certain carcinomas has been observed. In breast cancer, HSP27 expression has been shown to be responsive to estrogen levels. In both breast cancer and melanoma, high levels of HSP27 correlate with more aggressive tumors and decreased survival. Increased levels of HSP27 in the serum of patients with Chronic Obstructive Pulmonary Disease (COPD), breast cancer and melanoma may serve as a potential diagnostic marker. No crossreactivity is observed with HSP70, 90, 104 or 110.
Applications Reported: This STRSN antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, ELISA, and immunocytochemistry.
Applications Tested: This STRSN antibody has been tested by western blot of reduced HeLa cell lysate, immunocytochemistry on fixed and permeabilized A549 cells, and by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human tissue with either low or high pH antigen retreival at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; HS; HSP; HSP 27; HspB1; Phospho-HSP 27; SRP27; Stress-responsive protein 27
基因别名: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27
UniProt ID: (Human) P04792
Entrez Gene ID: (Human) 3315
retina homeostasis
regulation of translational initiation
negative regulation of protein kinase activity
movement of cell or subcellular component
response to unfolded protein
response to virus
regulation of autophagy
positive regulation of interleukin-1 beta production
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of mRNA stability
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
vascular endothelial growth factor receptor signaling pathway
platelet aggregation
negative regulation of protein serine/threonine kinase activity
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of endothelial cell chemotaxis
