Invitrogen

HSP70/HSC70 Monoclonal Antibody (BB70), APC

HSP70/HSC70 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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HSP70/HSC70 Antibody (MA5-45099) in IHC (PFA)

Immunohistochemistry analysis using inflamed colon. Fixation involved formalin. Samples were incubated with Hsp70 monoclonal antibody (Product # MA5-45099) at 1:10,000 for 12 hours at 4°C, followed by Biotin Goat Anti-Mouse at 1:2,000 for 1 hour at RT. Counterstain used was Mayer Hematoxylin (purple/blue) nuclear stain at 200 µL for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Infl... View More

HSP70/HSC70 Antibody in Western Blot (WB)

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产品信息

MA5-45099

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:200

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:2,000

免疫细胞化学 (ICC/IF)

1:200

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Bovine, Dog, Chicken, Fruit fly, Fish, Guinea pig, Hamster, Human, Mammal, Mouse, Sheep, Pig, Rabbit, Rat, Xenopus, Yeast

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

BB70

抗原

Chicken HSP70/HSP90 complex

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

47.35mM phosphate/2.15mM MES, pH 7.2, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2931553

产品详细信息

1 µg/mL of MA5-45099 was sufficient for detection of HSP70 and HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 72 (HSP) and approximately 73kDa (HSC).

靶标信息

HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a Protein Coding gene. Diseases associated with HSPA4 include Chagas Disease and Babesiosis. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Cellular responses to stimuli. An important paralog of this gene is HSPH1. HSPA8 (Heat Shock Protein Family A (Hsp70) Member 8) is a Protein Coding gene. Diseases associated with HSPA8 include Auditory System Disease and Borna Disease. Among its related pathways are Processing of Capped Intron-Containing Pre-mRNA and Selective autophagy. Gene Ontology (GO) annotations related to this gene include RNA binding and ubiquitin protein ligase binding. An important paralog of this gene is HSPA2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 5a; epididymis secretory sperm binding protein Li 72p; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 4; Heat shock 70 kDa protein 8; Heat shock 70-related protein APG-2; heat shock 70kd protein 10; heat shock 70kd protein 10 (HSC71); heat shock 70kD protein 4; heat shock 70kD protein 8; heat shock 70kDa protein 1-like; heat shock 70kDa protein 4; heat shock 70kDa protein 8; heat shock cognate 70; heat shock cognate 71 kD protein; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein Apg-2; heat shock protein cognate 70; Heat shock protein family A member 8; Heat shock protein family H member 2; heat shock protein, 110 kDa; Hsc70 ATPase; hsp70 RY; HSP70RY; HSPA4/HSPA8; Ischemia responsive 94 kDa protein; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; N-myristoyltransferase inhibitor protein 71; unnamed protein product

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基因别名: 2410008N15Rik; 70kDa; AI317151; APG-2; APG2; HEL-33; HEL-S-5a; HEL-S-72p; HS24/P52; HSC54; HSC70; hsc70.I; HSC71; Hsc73; Hsp110; HSP70; Hsp70RY; HSP71; HSP73; HSPA1; HSPA10; hspa1l; HSPA4; HSPA8; HSPH2; I79_004332; I79_022974; Irp94; LAP-1; LAP1; mKIAA4025; NIP71; RY; XELAEV_18037286mg; xhsp70