Invitrogen

HSP90 alpha Monoclonal Antibody (3B6)

高级验证

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

4个已发表图片

7 篇参考文献

查看其他21个HSP90 alpha抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

HSP90 alpha Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 18

HSP90 alpha Antibody (MA3-010) in ICC/IF

Immunofluorescence analysis of HSP90 alpha was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSP90 alpha Monoclonal Antibody(3B6) (Product # MA3-010) at 1:100 dilution in 0.1% BSA and incubated overnight ... View More

HSP90 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP90 alpha Antibody in Western Blot (WB)

HSP90 alpha Antibody in Immunohistochemistry (Frozen) (IHC (F))

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产品信息

MA3-010

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

Bovine, Human, Mouse, Non-human primate, Rabbit

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

3B6

抗原

Purified mouse HSP90.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites, PBS

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_325458

产品详细信息

MA3-010 detects heat shock protein 90 kDa (HSP90) from human, mouse, rat, and bovine tissues. This antibody does not detect chicken HSP90.

MA3-010 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects a 90 kDa protein representing HSP90 from MCF7 cell extract. MA3-011 is more sensitive in Western blot procedures and should be used when maximum sensitivity is desired. MA3-010 does not immunoprecipitate HSP90 in any form (free or complexed).

The MA3-010 antigen is purified mouse HSP90.

靶标信息

Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Heat Shock Protein 90 Involvement in the Development of Idiopathic Epiretinal Membranes.

Investigative ophthalmology & visual science

Tosi GM,Regoli M,Altera A,Galvagni F,Arcuri C,Bacci T,Elia I,Realini G,Orlandini M,Bertelli E

MA3-010 was used in Immunohistochemistry-immunofluorescence to characterise cells of idiopathic epiretinal membranes (iERMs).

Wed Jul 01 00:00:00 EDT 2020

Heat Shock Protein 90 Involvement in the Development of Idiopathic Epiretinal Membranes.

Investigative ophthalmology & visual science

Tosi GM,Regoli M,Altera A,Galvagni F,Arcuri C,Bacci T,Elia I,Realini G,Orlandini M,Bertelli E

MA3-010 was used in Immunohistochemistry-immunofluorescence to characterise cells of idiopathic epiretinal membranes (iERMs).

Wed Jul 01 00:00:00 EDT 2020

The distinct agonistic properties of the phenylpyrazolosteroid cortivazol reveal interdomain communication within the glucocorticoid receptor.

Molecular endocrinology (Baltimore, Md.)

Yoshikawa N,Yamamoto K,Shimizu N,Yamada S,Morimoto C,Tanaka H

MA3-010 was used in western blot to study the mechanism for differential actions of various synthetic steroids on glucocorticoid receptor.

Sun May 01 00:00:00 EDT 2005

Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane.

Reproductive biology and endocrinology : RB&E

Calvert ME,Digilio LC,Herr JC,Coonrod SA

MA3-010 was used in immunohistochemistry to identify highly abundant heat shock proteins and chaperone proteins in mature mouse egg proteome.

Fri Feb 14 00:00:00 EST 2003

Effect of stages of lactation on the concentration of a 90-kilodalton heat shock protein in bovine mammary tissue.

Journal of dairy science

Watanabe A,Miyamoto T,Katoh N,Takahashi Y

MA3-010 was used in western blot to investigate the differences of HSP90 expression in cow mammary tissue at various lactation stages

Wed Oct 01 00:00:00 EDT 1997

Agonist-free transformation of the glucocorticoid receptor in human B-lymphoma cells.

The Journal of steroid biochemistry and molecular biology

van den Berg JD,Smets LA,van Rooij H

MA3-010 was used in western blot to study the role of cell-cell interactions in the ligand-independent nuclear translocation of the glucocorticoid receptor

Thu Feb 01 00:00:00 EST 1996

High levels of non-activated receptors in glucocorticoid-sensitive S49wt mouse lymphoma cells incubated with dexamethasone.

The Journal of steroid biochemistry and molecular biology

van den Berg JD,Smets LA,Hutchison KA,van Rooij H,van den Elshout MM

MA3-010 was used in western blot to investigate the glucocorticoid receptor status of murine S49 lymphoma cells exposed to dexamethasone

Sat Oct 01 00:00:00 EDT 1994

ATP-dependent protein refolding activity in reticulocyte lysate. Evidence for the participation of different chaperone components.

FEBS letters

Nimmesgern E,Hartl FU

MA3-010 was used in western blot to investigate the role of chaperone components for energy-dependent protein refolding activity

Mon Sep 27 00:00:00 EDT 1993

生物信息学

蛋白别名: epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; Heat shock protein family C member 1; Heat shock protein HSP 90-alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HSP; HSP 86; HSP 90 alpha; HSP86; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen

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基因别名: 86kDa; 89kDa; AL024080; AL024147; EL52; HEL-S-65p; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2