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HSPA1B Antibody (PA5-28369) in ICC/IF
Immunofluorescence analysis of HSPA1B was performed using 70% confluent log phase PC-3 cells treated with Heat Shock (43 degree celsius for 1 hr). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSPA1B Polyclonal Antibody (Product # PA5-28369) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ S... View More
产品信息
PA5-28369
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein fragment corresponding to a region within amino acids 377 and 569 of Human HSP70 1B
偶联物
Unconjugated
形式
Liquid
浓度
0.55 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2545845
产品详细信息
Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS.
Predicted reactivity: Mouse (96%), Rat (98%), Xenopus laevis (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1B; heat shock 70kDa protein 1B; Heat shock protein; Heat shock protein family A member 1A; Heat shock protein family A member 1B; HSP; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70.1/HSP70.2; HSP70I
基因别名: HSP70-1B; HSP70-2; HSP70.2; HSP72; HSPA1; HSPA1A; HSPA1B; Hspa2; HSX70
UniProt ID: (Human) P0DMV8
Entrez Gene ID: (Human) 3304, (Rat) 294254
virus receptor activity
G-protein coupled receptor binding
receptor binding
protein binding
ATP binding
ATPase activity
enzyme binding
heat shock protein binding
ubiquitin protein ligase binding
ATPase activity, coupled
histone deacetylase binding
protein binding involved in protein folding
unfolded protein binding
C3HC4-type RING finger domain binding
molecular_function
double-stranded RNA binding
poly(A) RNA binding
protein N-terminus binding
positive regulation of gene expression
regulation of cell death
regulation of protein ubiquitination
negative regulation of protein ubiquitination
positive regulation of interleukin-8 production
cellular response to oxidative stress
cellular response to heat
protein refolding
ATP metabolic process
viral entry into host cell
protein stabilization
positive regulation of NF-kappaB transcription factor activity
negative regulation of cell death
cellular heat acclimation
positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway
negative regulation of inclusion body assembly
regulation of cellular response to heat
positive regulation of tumor necrosis factor-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
telomere maintenance
response to ischemia
DNA repair
mRNA catabolic process
response to unfolded protein
negative regulation of cell proliferation
response to radiation
response to heat
response to mechanical stimulus
neuron differentiation
negative regulation of cell growth
negative regulation of apoptotic process
response to ethanol
positive regulation of erythrocyte differentiation
positive regulation of neuron differentiation
