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Invitrogen
HSPA9 Monoclonal Antibody (30A5)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 7
HSPA9 Antibody (MA1-91639) in ICC/IF
Immunofluorescence analysis of HSPA9 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSPA9 Monoclonal Antibody (30A5) (Product # MA1-91639) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More
产品信息
MA1-91639
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Non-human primate,
Sheep,
Pig,
Rabbit,
Rat,
Xenopus,
Bovine,
Dog,
Guinea pig,
Hamster,
Human
已发表种属
Chicken,
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
30A5
抗原
Grp75 isolated from human Molt4 cells.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2120465
产品详细信息
Western blot detects a band ~75 kDa. A suggested positive control is HeLa cell lysate (heat shocked).
靶标信息
The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 70 kDa heat shock protein; 75 kDa glucose-regulated protein; C3H-specific antigen; catecholamine-regulated protein 40; epididymis secretory sperm binding protein Li 124m; GRP 75; GRP-75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9A; heat shock 70kDa protein 9B (mortalin-2); Heat shock protein; heat shock protein 9; heat shock protein 9A; heat shock protein cognate 74; Heat shock protein family A member 9; heat shock protein, 74 kDa, A; heat shock protein, A; HSP; MGC4500; Mortalin; mortalin, perinuclear; mortalin-2; mortalin2; MOT; mtHSP70; p66 MOT; p66-mortalin; PBP74; Peptide-binding protein 74; Stress-70 protein, mitochondrial
基因别名: 74kDa; CRP40; CSA; EVPLS; GRP-75; GRP75; HEL-S-124m; Hsc74; Hsp74; Hsp74a; HSPA9; hspa9-a; hspa9-b; Hspa9a; HSPA9B; I79_009139; Mortalin; MOT; Mot-2; MOT2; mt-HSP70; Mthsp70; MTHSP75; PBP74; SAAN; SIDBA4; XELAEV_18020021mg
UniProt ID: (Bovine) Q3ZCH0, (Human) P38646, (Chinese hamster) O35501, (Mouse) P38647
iron-sulfur cluster assembly
erythrocyte differentiation
regulation of erythrocyte differentiation
negative regulation of erythrocyte differentiation
negative regulation of hematopoietic stem cell differentiation
negative regulation of hemopoiesis
protein folding
protein export from nucleus
response to toxic substance
cellular response to interleukin-1
negative regulation of apoptotic process
