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Invitrogen
HYOU1 Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 10
HYOU1 Antibody (PA5-27655) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of HYOU1 was performed in U2OS cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HYOU1 Polyclonal Antibody (Product # PA5-27655) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
产品信息
PA5-27655
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Plant,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 695 and 999 of Human HYOU1
偶联物
形式
Liquid
浓度
0.41 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2545131
产品详细信息
Recommended positive controls: MCF-7, MDA-MB-231, mouse liver, rat brain.
Predicted reactivity: Mouse (92%), Rat (90%), Bovine (89%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
HYOU1 belongs to the heat shock protein 70 family. The protein is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 140 kDa Ca(2+)-binding protein; 150 kDa oxygen-regulated protein; 150-kDa oxygen regulated protein; 170 kDa glucose regulated protein GRP170; 170 kDa glucose-regulated protein; calcium binding protein 140; calcium binding protein, 140 kDa; CBP-140; DKFZp686N08236; FLJ94899; FLJ97572; Grp 170; GRP-170; Heat shock protein family H member 4; HSP 12A; Hypoxia up-regulated protein 1; Orp 150; ORP-150; oxygen regulated protein (150kD)
基因别名: AI415631; Cab140; CBP-140; GRP-170; GRP170; HSP12A; HSPH4; HYOU1; ORP-150; ORP150
UniProt ID: (Human) Q9Y4L1, (Rat) Q63617, (Mouse) Q9JKR6
Entrez Gene ID: (Human) 10525, (Rat) 192235, (Mouse) 12282
response to hypoxia
response to ischemia
response to endoplasmic reticulum stress
cellular response to hypoxia
negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
negative regulation of apoptotic process
negative regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway
ER to Golgi vesicle-mediated transport
receptor-mediated endocytosis
IRE1-mediated unfolded protein response
