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Histone H3 Antibody (A300-823A-M) in WB
Detection of human Histone H3 by western blot. Samples: Histones (5, 15 and 50 µg) isolated from HeLa cells. Antibodies: Affinity purified rabbit anti-Histone H3 antibody A300-823A-M (Lot A300-823A-M-2) used for WB at 1:1000. Detection: Chemiluminescence with exposure times of 10 seconds.
产品信息
A300-823A-M
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Between 100 and C-term
偶联物
形式
Liquid
浓度
200 µg/mL
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, D. melanogaster, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Rabbit, Pig and Orangutan
靶标信息
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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