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Reconstituted by adding 0.5 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
HRP, also known as horseradish peroxidase, is an enzyme that is commonly used in biochemistry and molecular biology research. It is derived from the root of the horseradish plant and catalyzes the oxidation of a wide range of substrates in the presence of hydrogen peroxide. HRP is widely used as a detection enzyme in immunoassays, such as ELISAs and Western blots, due to its high sensitivity and stability. It can also be used as a tracer in enzyme-linked immunosorbent assays (ELISAs) and as a labeling agent in fluorescent microscopy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Horseradish Peroxidase