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Invitrogen
Huntingtin Recombinant Rabbit Monoclonal Antibody (JB89-34)
高级验证
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
图: 1 / 16
Huntingtin Antibody (MA5-41256) in ICC/IF
Immunofluorescence of huntingtin was performed using DMS 53 WT and HTT KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Trito... View More
产品信息
MA5-41256
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JB89-34
抗原
Synthetic peptide within N-terminal Human Huntingtin
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2899009
靶标信息
Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HD protein; HD protein homolog; Huntingtin; huntingtin (Huntington disease); Huntington disease gene homolog; Huntington disease protein; huntington disease protein homolog
基因别名: AI256365; C430023I11Rik; HD; Hdh; HTT; IT15
UniProt ID: (Human) P42858
Entrez Gene ID: (Human) 3064, (Rat) 29424, (Mouse) 15194
urea cycle
citrulline metabolic process
establishment of mitotic spindle orientation
protein import into nucleus
mitochondrial transport
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
apoptotic process
mitochondrion organization
endoplasmic reticulum organization
Golgi organization
dopamine receptor signaling pathway
spermatogenesis
gastrulation
central nervous system development
brain development
cell aging
learning or memory
learning
grooming behavior
locomotory behavior
axo-dendritic transport
associative learning
determination of adult lifespan
visual learning
anatomical structure morphogenesis
anterior/posterior pattern specification
endosomal transport
lactate biosynthetic process from pyruvate
quinolinate biosynthetic process
striatum development
olfactory lobe development
neural plate formation
neurogenesis
peptide hormone secretion
insulin secretion
positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity
regulation of protein phosphatase type 2A activity
social behavior
hormone metabolic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
regulation of mitochondrial membrane permeability
vesicle transport along microtubule
regulation of synaptic plasticity
paraxial mesoderm formation
animal organ development
neuron development
mRNA transport
neuron apoptotic process
response to calcium ion
regulation of mitochondrial membrane potential
L-glutamate import
iron ion homeostasis
negative regulation of neuron death
negative regulation of cysteine-type endopeptidase activity
negative regulation of extrinsic apoptotic signaling pathway
vocal learning
positive regulation of cilium assembly
embryo development
