Invitrogen

IBA1 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13个已发表图片

12 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

IBA1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 36

IBA1 Antibody (PA5-21274) in ICC/IF

Immunofluorescence analysis of IBA1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IBA1 Polyclonal Antibody (Product # PA5-21274) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, 1:... View More

IBA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IBA1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

IBA1 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

IBA1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

IBA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of IBA1 was observed in THP-1 in comparison to A-431, HeLa and MCF7 using Anti-IBA1 Polyclonal Antibody (Product # PA5-21274) in Western Blot. {RE}

产品信息

PA5-21274

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看9篇已发表文章 9篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

流式细胞分析 (Flow)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region within amino acids 85 and 147 of Iba1 (Uniprot ID#P55008)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11156585

产品详细信息

Recommended positive controls: K562, THP-1, HL-60, mouse brain, mouse liver, rat liver, Mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

AIF1 is induced by cytokines and interferon. It is thought to be involved in the negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A role for P-selectin and complement in the pathological sequelae of germinal matrix hemorrhage.

Journal of neuroinflammation

Hatchell D,Alshareef M,Vasas T,Guglietta S,Borucki D,Guo C,Mallah K,Eskandari R,Tomlinson S

PA5-21274 was used in Immunohistochemistry-immunofluorescence to demonstrate vascular expression of the adhesion molecule P-selectin after GMH and investigate a strategy to specifically target complement inhibition to sites of P-selectin expression to mitigate the pathological sequelae of GMH.

Fri Jun 16 00:00:00 EDT 2023

In Utero Exposure to Zika Virus Results in sex-Specific Memory Deficits and Neurological Alterations in Adult Mice.

ASN neuro

Andrade TA,Fahel JS,de Souza JM,Terra AC,Souza DG,Costa VV,Teixeira MM,Bloise E,Ribeiro FM

Published figure using IBA1 polyclonal antibody (Product # PA5-21274) in Immunohistochemistry

Mon Aug 29 00:00:00 EDT 2022

VPS35 D620N knockin mice recapitulate cardinal features of Parkinson's disease.

Aging cell

Niu M,Zhao F,Bondelid K,Siedlak SL,Torres S,Fujioka H,Wang W,Liu J,Zhu X

PA5-21274 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to characterize the biochemical, pathological, and behavioral changes of a VPS35 D620N knockin (KI) mouse model with chronic aging.

Sat May 01 00:00:00 EDT 2021

VPS35 D620N knockin mice recapitulate cardinal features of Parkinson's disease.

Aging cell

Niu M,Zhao F,Bondelid K,Siedlak SL,Torres S,Fujioka H,Wang W,Liu J,Zhu X

Sat May 01 00:00:00 EDT 2021

Complement mediates neuroinflammation and cognitive decline at extended chronic time points after traumatic brain injury.

Acta neuropathologica communications

Mallah K,Couch C,Alshareef M,Borucki D,Yang X,Alawieh A,Tomlinson S

Published figure using IBA1 polyclonal antibody (Product # PA5-21274) in Immunohistochemistry

Tue Apr 20 00:00:00 EDT 2021

Complement mediates neuroinflammation and cognitive decline at extended chronic time points after traumatic brain injury.

Acta neuropathologica communications

Mallah K,Couch C,Alshareef M,Borucki D,Yang X,Alawieh A,Tomlinson S

Published figure using IBA1 polyclonal antibody (Product # PA5-21274) in Immunohistochemistry

Tue Apr 20 00:00:00 EDT 2021

METTL3-dependent RNA m⁶A dysregulation contributes to neurodegeneration in Alzheimer's disease through aberrant cell cycle events.

Molecular neurodegeneration

Zhao F,Xu Y,Gao S,Qin L,Austria Q,Siedlak SL,Pajdzik K,Dai Q,He C,Wang W,O'Donnell JM,Tang B,Zhu X

Published figure using IBA1 polyclonal antibody (Product # PA5-21274) in Immunohistochemistry

Thu Sep 30 00:00:00 EDT 2021

METTL3-dependent RNA m⁶A dysregulation contributes to neurodegeneration in Alzheimer's disease through aberrant cell cycle events.

Molecular neurodegeneration

Zhao F,Xu Y,Gao S,Qin L,Austria Q,Siedlak SL,Pajdzik K,Dai Q,He C,Wang W,O'Donnell JM,Tang B,Zhu X

Published figure using IBA1 polyclonal antibody (Product # PA5-21274) in Immunohistochemistry

Thu Sep 30 00:00:00 EDT 2021

Profound downregulation of neural transcription factor Npas4 and Nr4a family in fetal mice neurons infected with Zika virus.

PLoS neglected tropical diseases

Alpuche-Lazcano SP,Saliba J,Costa VV,Campolina-Silva GH,Marim FM,Ribeiro LS,Blank V,Mouland AJ,Teixeira MM,Gatignol A

PA5-21274 was used in Western Blotting to identify a profound dysregulation of neural transcription factors with an overexpression of miR-7013-5p that results in decreased Nr4a3 expression, likely a main contributor to ZIKV-induced neuronal dysfunction.

Sat May 01 00:00:00 EDT 2021

Loss of Diacylglycerol Kinase α Enhances Macrophage Responsiveness.

Frontiers in immunology

Manigat LC,Granade ME,Taori S,Miller CA,Vass LR,Zhong XP,Harris TE,Purow BW

PA5-21274 was used in Immunohistochemistry to test the hypothesis that DGKa, a known promoter of T-cell anergy, regulates macrophage activity in vivo and in vitro.

Fri Jan 07 00:00:00 EST 2022

AAV cis-regulatory sequences are correlated with ocular toxicity.

Proceedings of the National Academy of Sciences of the United States of America

Xiong W,Wu DM,Xue Y,Wang SK,Chung MJ,Ji X,Rana P,Zhao SR,Mai S,Cepko CL

PA5-21274 was used in Immunohistochemistry to suggest that ocular AAV toxicity is associated with certain AAV cis-regulatory sequences and/or their activity and that retinal damage occurs due to responses by the RPE and/or microglia.

Tue Mar 19 00:00:00 EDT 2019

Sensory Ganglia-Specific TNF Expression Is Associated With Persistent Nociception After Resolution of Inflammation.

Frontiers in immunology

Gonçalves WA,Rezende BM,de Oliveira MPE,Ribeiro LS,Fattori V,da Silva WN,Prazeres PHDM,Queiroz-Junior CM,Santana KTO,Costa WC,Beltrami VA,Costa VV,Birbrair A,Verri WA,Lopes F,Cunha TM,Teixeira MM,Amaral FA,Pinho V

Published figure using IBA1 polyclonal antibody (Product # PA5-21274) in Immunofluorescence

Mon Nov 16 00:00:00 EST 2020

Bilateral Implantation of Shear Stress Modifier in ApoE Knockout Mouse Induces Cognitive Impairment and Tau Abnormalities.

Frontiers in aging neuroscience

Nie S,Tan Y,Zhang Z,Chen G,Xiong J,Hu D,Ye K,Zhang Y,Cao X,Chen L,Zhang Z

PA5-21274 was used in Immunohistochemistry to investigate the mechanism underlying vascular cognitive impairment in an Apolipoprotein E knockout mouse model fed with western style food with implantation of bilateral shear stress modifiers.

Tue Sep 29 00:00:00 EDT 2020

Crocin Inhibits Oxidative Stress and Pro-inflammatory Response of Microglial Cells Associated with Diabetic Retinopathy Through the Activation of PI3K/Akt Signaling Pathway.

Journal of molecular neuroscience : MN

Yang X,Huo F,Liu B,Liu J,Chen T,Li J,Zhu Z,Lv B

PA521274 was used in western blot to assess the role of microglial cells in diabetic retinopathy

Sat Apr 01 00:00:00 EDT 2017

生物信息学

蛋白别名: AIF-1; Allograft inflammatory factor 1; balloon angioplasty responsive transcript; balloon angioplasty-responsive transcript 1; DADB-70P7.8; DASS-82G15.2; Em:AF129756.17; Iba; interferon gamma responsive transcript; ionized calcium binding adapter molecule 1; Ionized calcium-binding adapter molecule 1; Microglia response factor; MRF-1; protein BART-1; Protein G1; testis specific

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基因别名: AI607846; AIF-1; AIF1; BART-1; Bart1; D17H6S50E; G1; IBA1; IRT-1; IRT1; mrf-1; Mrf1

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UniProt ID: (Human) P55008, (Mouse) O70200, (Rat) P55009

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Entrez Gene ID: (Human) 199, (Mouse) 11629, (Rat) 29427

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功能

molecular_function calcium ion binding actin filament binding actin binding protein binding metal ion binding calmodulin-related

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