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Invitrogen
IFIT1 Monoclonal Antibody (OTI3G8)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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IFIT1 Antibody (MA5-25050) in ICC/IF
Immunofluorescence analysis of Interferon-induced protein with tetratricopeptide repeats 1 was performed using 70% confluent log phase HeLa treated with Human IFN Beta (1000 U/mL for 16 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IFIT1 Monoclonal Antibody (OTI3G8) (Product # MA5-25050) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with... View More
产品信息
MA5-25050
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
OTI3G8
抗原
Full length human recombinant protein of IFIT1 produced in HEK293T cell
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 1% BSA, 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2724504
靶标信息
Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: IFI-56K; IFIT-1; interferon, alpha-inducible protein (MW 56kD); Interferon-induced 56 kDa protein; Interferon-induced protein with tetratricopeptide repeats 1; interferon-inducible mRNA 561
基因别名: C56; G10P1; IFI-56; IFI-56K; IFI56; IFIT-1; IFIT1; IFNAI1; ISG56; P56; RNM561
UniProt ID: (Human) P09914
Entrez Gene ID: (Human) 3434
response to virus
intracellular transport of viral protein in host cell
negative regulation of protein binding
positive regulation of viral genome replication
negative regulation of viral genome replication
negative regulation of defense response to virus by host
negative regulation of helicase activity
defense response to virus
type I interferon signaling pathway
cellular response to type I interferon
cellular response to exogenous dsRNA
