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Invitrogen
IFIT1 Polyclonal Antibody
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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IFIT1 Antibody (PA5-31254) in WB
Western blot was performed using Anti-IFIT1 Polyclonal Antibody (Product # PA5-31254) and a 55 kDa band corresponding to Interferon-induced protein with tetratricopeptide repeats 1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HT-1080 (Lane 1), HT-1080 treated with Human IFN Beta (1000U/mL for 24 hrs) (Lane 2), HeLa (Lane 3) and HeLa treated with Human IFN Beta (1000U/mL for 24 hrs) (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transf... View More
产品信息
PA5-31254
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 36 and 281 of Human IFIT1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2548728
产品详细信息
Recommended positive controls: HeLa, HeLa(10 ng/mL hIFN-gamma1 treatment for 16hr).
Predicted reactivity: Chimpanzee (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: IFI-56K; IFIT-1; interferon, alpha-inducible protein (MW 56kD); Interferon-induced 56 kDa protein; Interferon-induced protein with tetratricopeptide repeats 1; interferon-inducible mRNA 561
基因别名: C56; G10P1; IFI-56; IFI-56K; IFI56; IFIT-1; IFIT1; IFNAI1; ISG56; P56; RNM561
UniProt ID: (Human) P09914
Entrez Gene ID: (Human) 3434
response to virus
intracellular transport of viral protein in host cell
negative regulation of protein binding
positive regulation of viral genome replication
negative regulation of viral genome replication
negative regulation of defense response to virus by host
negative regulation of helicase activity
defense response to virus
type I interferon signaling pathway
cellular response to type I interferon
cellular response to exogenous dsRNA
