Invitrogen

IFNAR2 Monoclonal Antibody (MMHAR-2)

高级验证

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

8 篇参考文献

查看其他21个IFNAR2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
高级验证 (1)

IFNAR2 Antibody in Functional Assay (Functional)

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IFNAR2 Antibody (213851) in Functional

THP-1 cells were infected with virus in presence of IFNAR2 antibody (Product # 213851, final concentration: 10 µg/mL). Cells were collected at 24 hours post-infection followed by RNA extraction and RT-qPCR. The mRNA level of AIM2, an interferon-inducible protein, was measured by real-time PCR. Data courtesy of Antibody Data Exchange Program.

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产品信息

213851

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

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中和实验 (Neu)

Assay-dependent

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功能检测 (Functional)

10 µg/mL

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抑制试剂 (IA)

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阻断反应 (BLOCK)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

MMHAR-2

抗原

Human interferon alpha/beta receptor chain 2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ion-exchange chromatography, Size-exclusion chromatography

保存液

PBS with 0.1% BSA

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Dry ice

RRID

AB_223508

产品详细信息

This antibody neutralizes human IFN-alpha receptor, interacting with the extracellular domain. It binds to human IFN-alpha receptor with high affinity and blocks biological action of Type I IFNs. To measure bioactivity, interferon was titrated with the use of a cytopathic effect inhibition assay, where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha to a 50% endpoint. In this antiviral assay, approximately one unit/mL of interferon was necessary to produce a cytopathic effect of 50%.

Purity is >95% by SDS-PAGE and endotoxin level <1 EU/ug.

Store at -20ºC for up to 6 months. For long-term storage, store at -70ºC for retention of full activity. Thaw product by incubation in cold water and aliquot into separate vials, adding 0.1% BSA for additional stability. Refreeze product using a dry ice or a dry ice/alcohol bath.

Packaged by and distributed for PBL Assay Science

靶标信息

The type I interferons, IFN-alpha and IFN-beta, are a group of structurally and functionally related proteins that are induced by either viruses or double-stranded RNA and are defined by their ability to confer an antiviral state in cells. IFN-alpha and IFN-beta appear to compete with one another for binding to a common cell surface receptor, while immune IFN (IFN-gamma) binds to a distinct receptor. This distinct receptor, IFN-alphaR, is only weakly responsive to type I interferons, in contrast to IFN-alpha/betaR, which binds to and responds effectively to IFN-beta and to several of the IFN-alpha subtypes. IFN-alpha/betaR is expressed as two alternatively spliced transcripts, designated IFN-alpha/betaRalpha (IFN-alpha/betaR1) and IFN-alpha/betaRbeta (IFN-alpha/betaR2), both of which are involved in signal transduction and ligand binding.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages.

eLife

Dupont M,Souriant S,Balboa L,Vu Manh TP,Pingris K,Rousset S,Cougoule C,Rombouts Y,Poincloux R,Ben Neji M,Allers C,Kaushal D,Kuroda MJ,Benet S,Martinez-Picado J,Izquierdo-Useros N,Sasiain MDC,Maridonneau-Parini I,Neyrolles O,Vérollet C,Lugo-Villarino G

213851 was used in inhibition assays to uncover a deleterious role for Siglec-1 in TB-HIV-1 co-infection and open new avenues to understand TNT biology.

Mon Mar 30 00:00:00 EDT 2020

Human monocyte-derived macrophages inhibit HCMV spread independent of classical antiviral cytokines.

Virulence

Becker J,Kinast V,Döring M,Lipps C,Duran V,Spanier J,Tegtmeyer PK,Wirth D,Cicin-Sain L,Alcamí A,Kalinke U

213851 was used in Flow cytometry/Cell sorting to study the mechanisms that restrict the spread of human cytomegalovirus (HCMV).

Thu Apr 18 00:00:00 EDT 2019

PARP1 depletion induces RIG-I-dependent signaling in human cancer cells.

PloS one

Ghosh R,Roy S,Franco S

213851 was used in Inhibition Assays to demonstrate pattern recognition receptor RIG-I signalling in human cancer cells is induced by single strand break repair protein PARP1 depletion.

Tue Jul 24 00:00:00 EDT 2018

Targeting type I interferon-mediated activation restores immune function in chronic HIV infection.

The Journal of clinical investigation

Zhen A,Rezek V,Youn C,Lam B,Chang N,Rick J,Carrillo M,Martin H,Kasparian S,Syed P,Rice N,Brooks DG,Kitchen SG

213851 was used in blocking or activating experiment to study the restoration of immune function in chronic HIV infection

Tue Jan 03 00:00:00 EST 2017

Priming and Activation of Inflammasome by Canarypox Virus Vector ALVAC via the cGAS/IFI16-STING-Type I IFN Pathway and AIM2 Sensor.

Journal of immunology (Baltimore, Md. : 1950)

Liu F,Niu Q,Fan X,Liu C,Zhang J,Wei Z,Hou W,Kanneganti TD,Robb ML,Kim JH,Michael NL,Sun J,Soong L,Hu H

THP-1 cells were infected with ALVAC in the presence of IFNAR2 antibody (product# 213851, final concentration: 10ug/ml). The mRNA level of AIM2, an interferon-inducible protein, was reduced in the presence of IFNAR2 antibody.

Wed Nov 01 00:00:00 EDT 2017

Endothelial IL-33 Expression Is Augmented by Adenoviral Activation of the DNA Damage Machinery.

Journal of immunology (Baltimore, Md. : 1950)

Stav-Noraas TE,Edelmann RJ,Poulsen LC,Sundnes O,Phung D,Küchler AM,Müller F,Kamen AA,Haraldsen G,Kaarbø M,Hol J

213851 was used in blocking or activating experiment to identify factors that contribute to adenoviral transduction stimulation of IL-33 in endothelial cells

Sat Apr 15 00:00:00 EDT 2017

Replication of Human Norovirus RNA in Mammalian Cells Reveals Lack of Interferon Response.

Journal of virology

Qu L,Murakami K,Broughman JR,Lay MK,Guix S,Tenge VR,Atmar RL,Estes MK

213851 was used in blocking or activating experiment to assess the effect of the IFN response on human noroviruses

Sat Oct 01 00:00:00 EDT 2016

Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.

PLoS pathogens

Bowers NL,Helton ES,Huijbregts RP,Goepfert PA,Heath SL,Hel Z

213851 was used in blocking or activating experiment to characterize the role of PD-L1/PD-1 pathway in HIV-1 infection due to immune suppression by neutrophils

Sat Mar 01 00:00:00 EST 2014

生物信息学

蛋白别名: human interferon alpha/beta receptor; IFN alpha/betaRbeta; IFN R2; IFN-alpha/beta receptor 2; IFN-alpha/betaR2; IFN-alpha/betaRbeta; IFN-R-2; IFNalpha/beta R2; IFNalpha/beta-Rbeta; IFNalpha/betaR2; IFNR2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; Interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2

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基因别名: IFN-alpha-REC; IFN-R; IFNABR; IFNAR2; IFNARB; IMD45

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UniProt ID: (Human) P48551

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Entrez Gene ID: (Human) 3455

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功能

type I interferon receptor activity protein binding protein kinase binding interleukin-20 binding transmembrane signal receptor

参与通路

regulation of transcription from RNA polymerase II promoter cell surface receptor signaling pathway JAK-STAT cascade cell proliferation response to virus response to interferon-alpha response to interferon-beta defense response to virus type I interferon signaling pathway regulation of type I interferon-mediated signaling pathway