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IGF2 Antibody (500-P12-1MG) in WB
Western Blot: To detect Human IGF-II by Western Blot analysis IGF2 Polyclonal Antibody (Product # 500-P12-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P12-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human IGF-II
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929328
产品详细信息
AA Sequence of recombinant protein: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-II. Anti-Human IGF-II specific antibody was purified by affinity chromatography employing an immobilized Human IGF-II matrix.
Sandwich ELISA: To detect Human IGF-II by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IGF-II (500-P12Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-II.
Western Blot: To detect Human IGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: FLJ22066; FLJ44734; H-IGF-2; IGF; IGF-II; Insulin like growth factor; insulin-like growth factor 2 (somatomedin A); Insulin-like growth factor II; insulin-like growth factor type 2; preptin; Somatomedin-A; T3M-11-derived growth factor
基因别名: C11orf43; GRDF; IGF-II; IGF2; PP1446; PP9974
UniProt ID: (Human) Q9UC68
Entrez Gene ID: (Human) 3481
skeletal system development
ossification
positive regulation of protein phosphorylation
platelet degranulation
glucose metabolic process
regulation of gene expression by genetic imprinting
regulation of transcription, DNA-templated
multicellular organism development
female pregnancy
memory
positive regulation of cell proliferation
insulin receptor signaling pathway
response to radiation
response to nutrient levels
response to estradiol
response to nicotine
insulin receptor signaling pathway via phosphatidylinositol 3-kinase
wound healing
positive regulation of activated T cell proliferation
response to drug
positive regulation of catalytic activity
positive regulation of MAPK cascade
cellular protein metabolic process
response to ethanol
positive regulation of glycogen biosynthetic process
positive regulation of mitotic nuclear division
negative regulation of natural killer cell mediated cytotoxicity
positive regulation of insulin receptor signaling pathway
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of cell division
positive regulation of protein kinase B signaling
cellular response to mechanical stimulus
positive regulation of protein serine/threonine kinase activity
positive regulation of receptor activity
positive regulation of glycogen (starch) synthase activity
