Invitrogen

IGF2R Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2个已发表图片

7 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

IGF2R Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

IGF2R Antibody (PA3-850) in ICC/IF

Immunofluorescent analysis of CI-MPR (green) showing staining in the cytoplasm and nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CI-MPR polyclonal antibody (Product # PA3-850) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a Dy... View More

Product Image Gallery

IGF2R Antibody — alpha-SMA fibril formation in primary human corneal myofibroblasts following transduction with control (pLKO) or shRNA-IGF2R lentiviral particles (KD). Lentiviral particle transduced or nontransduced cells (WT) were cultured in 1 ng/mL TGF-beta1 for 7 days under serum-free conditions. (A) Lysates were generated and subjected to Western blot analysis (equivalent cell numbers loaded into each lane). Membranes were probed with IGF2R-, alpha-SMA-, or LAMP1-specific antibodies. Quantification is shown below the representative blots and is derived from corneal cultures obtained from four independent donors. Data are plotted as a percentage of the intensity of IGF2R, alpha-SMA, or LAMP1 bands in the shRNAi-IGF2R KD cells and cells transduced with pLKO control vector relative to nontransduced WT cells set to 100%. Values represent the mean SE. The Student's t-test was used to compare the IGF2R KD cells to the pLKO control cells. *P < 0.02, n = 4. (B) Fixed cells were incubated with antibody specific to alpha-SMA that was visualized using a goat anti-mouse secondary antibody conjugated to Alexa Fluor 568 (red) and FITC-conjugated phalloidin (green). Nuclei were stained with Hoescht 33342 dye. An overlay of the phalloidin and alpha-SMA-stained cells is shown in the bottom. Representative micrographs are shown. Scale bar: 100 µm. (C) The images from the studies shown in (D) were quantified for the number of cells containing alpha-SMA fibrils. Approximately 200 to 500 cells per micrograph were scored for the presence of alpha-SMA-containing fibrils. {KD}

产品信息

PA3-850

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:3,000

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:50-1:500

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点杂交 (DB)

1:1,000

产品规格

种属反应

Bovine, Human, Mouse, Non-human primate

已发表种属

Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

300 kDa cation-independent receptor purified from adult bovine liver tissue.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

whole serum

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2138964

产品详细信息

PA3-850 detects cation-independent mannose-6-receptor(CI-MPR) in human, bovine, mouse and non-human primate cells.

PA3-850 has been successfully used in ICC/IF, Dot blot and Western blotting procedures. By Western blot, it detects a 300 kDa protein representing CI-MPR under non-reducing conditions.

The PA3-850 immunogen is a 300 kDa cation-independent receptor purified from adult Bovine liver tissue.

靶标信息

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of approximately 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and approximately 10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An approximately 240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility.

Frontiers in cell and developmental biology

Bilekova S,Garcia-Colomer B,Cebrian-Serrano A,Schirge S,Krey K,Sterr M,Kurth T,Hauck SM,Lickert H

PA3-850 was used in Immunohistochemistry (Frozen) to support our hypothesis of inceptor as a universal lysosomal or lysosome-related organelle sorting receptor expressed in several secretory tissues.

Wed Sep 13 00:00:00 EDT 2023

Tumor protein D54 defines a new class of intracellular transport vesicles.

The Journal of cell biology

Larocque G,La-Borde PJ,Clarke NI,Carter NJ,Royle SJ

Published figure using IGF2R polyclonal antibody (Product # PA3-850) in Immunocytochemistry

Mon Jan 06 00:00:00 EST 2020

Chlamydia trachomatis CT229 Subverts Rab GTPase-Dependent CCV Trafficking Pathways to Promote Chlamydial Infection.

Cell reports

Faris R,Merling M,Andersen SE,Dooley CA,Hackstadt T,Weber MM

PA3-850 was used in Immunocytochemistry to study the role of CT229 as a regulator of multiple host vesicular trafficking pathways essential for chlamydial infection.

Tue Mar 19 00:00:00 EDT 2019

Arf6 controls retromer traffic and intracellular cholesterol distribution via a phosphoinositide-based mechanism.

Nature communications

Marquer C,Tian H,Yi J,Bastien J,Dall'Armi C,Yang-Klingler Y,Zhou B,Chan RB,Di Paolo G

PA3-850 was used in immunocytochemistry to study a phosphoinositide-based mechanism that Arf6 uses to control retromer traffic and intracellular cholesterol distribution

Thu Jun 23 00:00:00 EDT 2016

Expression of insulin-like growth factor 2 receptor in corneal keratocytes during differentiation and in response to wound healing.

Investigative ophthalmology & visual science

Bohnsack RN,Warejcka DJ,Wang L,Gillespie SR,Bernstein AM,Twining SS,Dahms NM

PA3-850 was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to study corneal keratocytes and expression of insulin-like growth factor 2 receptor during wound healing response and differentiation

Thu Oct 30 00:00:00 EDT 2014

Expression of insulin-like growth factor 2 receptor in corneal keratocytes during differentiation and in response to wound healing.

Investigative ophthalmology & visual science

Bohnsack RN,Warejcka DJ,Wang L,Gillespie SR,Bernstein AM,Twining SS,Dahms NM

Thu Oct 30 00:00:00 EDT 2014

Identification of novel murine- and human-specific RPGRIP1 splice variants with distinct expression profiles and subcellular localization.

Investigative ophthalmology & visual science

Lu X,Ferreira PA

PA3-850 was used in immunocytochemistry to investigate the expression profiles of RPGRIP1 isoforms and their respective cellular distribution

Wed Jun 01 00:00:00 EDT 2005

生物信息学

蛋白别名: 300 kDa mannose 6-phosphate receptor; cation-independent mannose 6-phosphate receptor; cation-independent mannose 6-phosphate receptor protein; Cation-independent mannose-6-phosphate receptor; cation-independent MPR; CD222; CI Man-6-P receptor; IGF-II receptor; IGF-II/CI-MPR; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; insulin-like growth factor receptor type 2; M6P/IGF2 receptor; M6P/IGF2R; M6PR; mannose-6-phosphate receptor, cation independent; MPR 300

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基因别名: AI661837; CD222; CI-M6PR; CI-MPR; CIMPR; IGF2R; M6P; M6P-R; M6P/IGF2R; MPR 300; MPR1; MPR300; MPRI

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UniProt ID: (Human) P11717, (Bovine) P08169, (Mouse) Q07113

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Entrez Gene ID: (Human) 3482, (Bovine) 281849, (Mouse) 16004

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功能