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Invitrogen

IGFBP5 Polyclonal Antibody

1个已发表图片

5 篇参考文献

查看其他12个IGFBP5抗体

货号 PA5-37369

飞享价 CNY 4,457.00 6,145.00

活动截止日期 31-Dec-2025

100 µL

批量或定制请求

抗体检测数据 (2)
已发表图片 (1)

Application

IGFBP5 Antibody in Western Blot (WB)

图: 1 / 3

IGFBP5 Antibody (PA5-37369) in WB

Western blot analysis of IGFBP5. Samples were incubated with IGFBP5 polyclonal antibody (Product # PA5-37369).

IGFBP5 Antibody in Western Blot (WB)

IGFBP5 Antibody in Western Blot (WB)

IGFBP5 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

IGFBP5 Antibody in Western Blot (WB) — Western blot analysis of IGFBP5. Samples were incubated with IGFBP5 polyclonal antibody (Product # PA5-37369).

IGFBP5 Antibody in Western Blot (WB) — Western blot analysis of IGFBP5 using IGFBP5 polyclonal antibody (Product # PA5-37369).

IGFBP5 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 7. Senescence independent Tff3 knockdown induces IGFBP5, leading to attenuation of IGF signaling. A, Western blot analysis for the expression of IGFBP5 and pIGFR1 using protein extracts from immortalized mouse BNL CL.2 liver cells with stable Tff3 knockdown ( shTff3 A and shTff3 B ) or control shRNA (shNC). GFP was used as a loading control because stable expression of shRNAs (shNC, shTff3 A , and shTff3 B ) was linked to the expression of GFP. B, Western blot analysis for the expression status of IGFBP5 and pIGFR1 using protein extracts from human liver cancer cell line (HepG2) with stable TFF3 knockdown ( shTFF3 huA and shTFF3 huB ) or control shRNA (shNC). Housekeeping gene alpha-tubulin was used as loading control. C, Western blot analysis for the secreted status of IGFBP5 protein using the supernatant from HepG2 cells expressing either shRNA against TFF3 ( shTFF3 huA ) or control shRNA (shNC). Secreted AFP was used as loading control. D, Immunofluorescence staining against IGFBP5 using HepG2 cells expressing either shTFF3 huA or shNC (x200 magnification). Figure 7. Senescence independent Tff3 knockdown induces IGFBP5, leading to attenuation of IGF signaling. A, Western blot analysis for the expression of IGFBP5 and p-IGFR1 using protein extracts from immortalized mouse BNL CL.2 liver cells with stable Tff3 knockdown (shTff3 A and shTff3 B) or control shRNA (shNC). GFP was used as a loading control because stable expression of shRNAs (shNC, shTff3 A, and shTff3 B)

产品信息

PA5-37369

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

-

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

-

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:200

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant full length human IGFBP5

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2554033

产品详细信息

This antibody detects endogenous protein at a molecular weight of 31 kDa.

Purity is >95% by SDS-PAGE.

靶标信息

The insulin-like growth factor-binding proteins, or IGFBPs, are a family of homologous proteins that have co-evolved with the IGFs. They serve not only as shuttle molecules for the soluble IGFs, but also confer a level of regulation to the IGF signaling system. Physical association of the IGFBPs with IGF influences the bio-availability of the growth factors, as well as their concentration and distribution in the extracellular environment. In addition, the IGFBPs appear to have biological activity independent of the IGFs. Seven IGFBPs have thus far been described, each differing in their tissue distribution, half-lives and modulation of IGF interactions with their receptors. For instance, IGFBP1 is negatively regulated by insulin production. The IGFBP1 gene is expressed at a high level during fetal liver development and in response to nutritional changes and diabetes. It has been suggested that IGFBP2 functions as chaperone, escorting IGFs to their target tissues. It is expressed in several human tissues including fetal eye and fetal brain. IGFBP3 is the most abundant IGFBP and is complexed with roughly 80% of the serum IGFs. Both IGFBP3 and IGFBP4 are released by dermal fibroblasts in response to incision injury. IGFBP5 is secreted by myoblasts and may play a key role in muscle differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-37369 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor development.

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-37369 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor development.

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-37369 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor development.

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-37369 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor development.

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-37369 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor development.

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-37369 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor development.

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-37369 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor development.

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-37369 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor development.

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-37369 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor development.

Fri Feb 03 00:00:00 UTC 2023

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes.

Cancer research

Wuestefeld A,Iakovleva V,Yap SXL,Ong ABL,Huang DQ,Shuen TWH,Toh HC,Dan YY,Zender L,Wuestefeld T

PA5-37369 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to investigate early transformation events in liver cancer and the role of premalignant, senescent hepatocytes in hepatocellular tumor development.

Fri Feb 03 00:00:00 UTC 2023

EZH1 and EZH2 promote skeletal growth by repressing inhibitors of chondrocyte proliferation and hypertrophy.

Nature communications

Lui JC,Garrison P,Nguyen Q,Ad M,Keembiyehetty C,Chen W,Jee YH,Landman E,Nilsson O,Barnes KM,Baron J

PA537369 was used in immunohistochemistry - paraffin section to discover a role for H3K27 methylation in governing chondrocyte proliferation and hypertrophy in the growth plate

Tue Nov 29 00:00:00 UTC 2016

生物信息学

蛋白别名: IBP 5; IBP-5; IGF BP5; IGF-binding protein 5; IGFBP; IGFBP 5; IGFBP-5; Insulin like growth factor binding protein; insulin-like growth factor binding protein 5; Insulin-like growth factor-binding protein 5

基因别名: IBP5; IGF-BP5; Igfbp-5; IGFBP5

UniProt ID: (Human) P24593, (Rat) P24594

Entrez Gene ID: (Human) 3488, (Rat) 25285

fibronectin binding protein binding insulin-like growth factor I binding insulin-like growth factor II binding insulin-like growth factor binding protease inhibitor

参与通路

regulation of cell growth osteoblast differentiation glucose metabolic process signal transduction female pregnancy regulation of glucose metabolic process negative regulation of smooth muscle cell migration negative regulation of translation negative regulation of cell migration hair follicle morphogenesis intracellular signal transduction glucose homeostasis positive regulation of insulin-like growth factor receptor signaling pathway negative regulation of insulin-like growth factor receptor signaling pathway cellular protein metabolic process type B pancreatic cell proliferation negative regulation of osteoblast differentiation negative regulation of growth lung alveolus development skeletal muscle tissue growth negative regulation of smooth muscle cell proliferation striated muscle cell differentiation positive regulation of protein kinase B signaling mammary gland involution response to growth hormone cellular response to cAMP cellular response to organic cyclic compound negative regulation of muscle tissue development negative regulation of skeletal muscle hypertrophy regulation of growth regulation of insulin-like growth factor receptor signaling pathway