Invitrogen

IL-1 alpha Polyclonal Antibody, PeproTech®

IL-1 alpha Antibody in Western Blot (WB)

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IL-1 alpha Antibody (500-P180G-1MG) in WB

Western Blot: To detect Rat IL-1alpha by Western Blot analysis IL-1 alpha Polyclonal Antibody (Product # 500-P180G-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P180G-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

其他PubMed (Misc)

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产品规格

种属反应

Rat

已发表种属

Rat

宿主/亚型

Goat

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Rat IL-1alpha

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2929676

产品详细信息

AA Sequence of recombinant protein: MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat IL-1alpha. Anti-Rat IL-1alpha-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-1alpha matrix.

Sandwich ELISA: To detect Rat IL-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-1alpha (500-P180GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-1alpha.

Western Blot: To detect Rat IL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

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