Invitrogen

IL-1 beta Monoclonal Antibody (1400.24.17), Biotin

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IL-1 beta Antibody (MM425BB) in ELISA

Proceedings of the National Academy of Sciences of the United States of America 2005 - Published figure using IL-1 beta monoclonal antibody (Product # MM425BB) in ELISA

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产品信息

MM425BB

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化 (IHC)

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酶联免疫吸附实验 (ELISA)

Assay-dependent

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中和实验 (Neu)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1400.24.17

抗原

Recombinant mouse IL-1 beta

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS with 4% BSA

内含物

proprietary preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_223603

产品详细信息

MM425BB targets IL-1 beta in ELISA, and WB applications and shows reactivity with mouse samples.

The MM425BB immunogen is recombinant mouse IL-1 beta.

MM425BB detects IL-1 beta which has a predicted molecular weight of approximately 17 kDa.

靶标信息

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Distinct regulation by lipopolysaccharides of the expression of interleukin-1β by murine macrophages and salivary glands.

Innate immunity

Seil M,El Ouaaliti M,Abdou Foumekoye S,Pochet S,Dehaye JP

MM425BB was used in an ELISA assay to conclude that IL-1Β is constitutively expressed by submandibular glands and its secretion is not regulated by a P2X(7) agonist.

Wed Feb 01 00:00:00 EST 2012

Inflammasome-mediated production of IL-1beta is required for neutrophil recruitment against Staphylococcus aureus in vivo.

Journal of immunology (Baltimore, Md. : 1950)

Miller LS,Pietras EM,Uricchio LH,Hirano K,Rao S,Lin H,O'Connell RM,Iwakura Y,Cheung AL,Cheng G,Modlin RL

MM425BB was used in immunohistochemistry to study the effect of interleukin 1 beta on protection against Staphylococcus aureus

Thu Nov 15 00:00:00 EST 2007

Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness.

Proceedings of the National Academy of Sciences of the United States of America

Johnson DR,O'Connor JC,Dantzer R,Freund GG

Published figure using IL-1 beta monoclonal antibody (Product # MM425BB) in ELISA

Tue Oct 18 00:00:00 EDT 2005

IL-1beta-mediated innate immunity is amplified in the db/db mouse model of type 2 diabetes.

Journal of immunology (Baltimore, Md. : 1950)

O'Connor JC,Satpathy A,Hartman ME,Horvath EM,Kelley KW,Dantzer R,Johnson RW,Freund GG

MM425BB was used in ELISA to study the interleukin 1beta-mediated innate immunity in the db/db mouse model of type 2 diabetes

Fri Apr 15 00:00:00 EDT 2005

Stimulus-dependent requirement for granulocyte-macrophage colony-stimulating factor in inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Cook AD,Braine EL,Hamilton JA

MM425BB was used in ELISA to investigate the role of granulocyte-macrophage colony-stimulating factor in inflammation

Fri Oct 01 00:00:00 EDT 2004

Sleep-deprived mice show altered cytokine production manifest by perturbations in serum IL-1ra, TNFa, and IL-6 levels.

Brain, behavior, and immunity

Hu J,Chen Z,Gorczynski CP,Gorczynski LY,Kai Y,Lee L,Manuel J,Gorczynski RM

MM425BB was used in ELISA to investigate the changes of cytokine expression in mice with sleep disruption

Mon Dec 01 00:00:00 EST 2003

Essential role for Ca2+ in regulation of IL-1beta secretion by P2X7 nucleotide receptor in monocytes, macrophages, and HEK-293 cells.

American journal of physiology. Cell physiology

Gudipaty L,Munetz J,Verhoef PA,Dubyak GR

MM425BB was used in ELISA to investigate the role of calcium ion in P2X7 nucleotide receptor-mediated interleukin 1 beta secretion

Fri Aug 01 00:00:00 EDT 2003

Mediators of peripheral blood neutrophilia induced by photodynamic therapy of solid tumors.

Cancer letters

Cecic I,Korbelik M

MM425BB was used in blocking/activating experiment to investigate the effect of photodynamic therapy on peripheral blood neutrophilia

Sun Sep 08 00:00:00 EDT 2002

The CY domain of the Fcgamma RIa alpha-chain (CD64) alters gamma-chain tyrosine-based signaling and phagocytosis.

The Journal of biological chemistry

Edberg JC,Qin H,Gibson AW,Yee AM,Redecha PB,Indik ZK,Schreiber AD,Kimberly RP

MM425BB was used in ELISA to study the mechanism for the gamma-chain tyrosine-based signaling and phagocytosis

Fri Oct 25 00:00:00 EDT 2002

IFN-gamma inhibits lipopolysaccharide-induced interleukin-1 beta in primary murine macrophages via a Stat1-dependent pathway.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

De Boer ML,Hu J,Kalvakolanu DV,Hasday JD,Cross AS

MM425BB was used in ELISA to study the role of interferon-gamma in LPS-induced interleukin-1beta in primary murine macrophages

Sun Jul 01 00:00:00 EDT 2001

Development of an animal model for neurocysticercosis: immune response in the central nervous system is characterized by a predominance of gamma delta T cells.

Journal of immunology (Baltimore, Md. : 1950)

Cardona AE,Restrepo BI,Jaramillo JM,Teale JM

MM425BB was used in immunohistochemistry to develop a neurocysticercosis animal model

Fri Jan 15 00:00:00 EST 1999

生物信息学

蛋白别名: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta

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基因别名: Il-1b; IL-1beta; Il1b

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UniProt ID: (Mouse) P10749

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Entrez Gene ID: (Mouse) 16176

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功能

receptor binding cytokine activity interleukin-1 receptor binding protein domain specific binding interleukin superfamily

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