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IL-10 Antibody (PA5-94918) in IHC (P)
Immunohistochemistry analysis of IL-10 in paraffin-embedded section of rat spleen tissue. Samples were incubated with IL-10 polyclonal antibody (Product # PA5-94918) using a dilution of 2 μg/mL overnight at 4°C followed by Peroxidase Conjugated Goat Anti-rabbit IgG incubated for 30 minutes at 37°C. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue ... View More
产品信息
PA5-94918
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived rat IL-10 recombinant protein (Position: S19-N178).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2806724
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1
基因别名: Il-10; Il10; IL10X
UniProt ID: (Rat) P29456
Entrez Gene ID: (Rat) 25325
negative regulation of cytokine production
response to molecule of bacterial origin
negative regulation of cytokine secretion involved in immune response
negative regulation of chronic inflammatory response to antigenic stimulus
positive regulation of B cell apoptotic process
inflammatory response
immune response
aging
response to organic substance
regulation of gene expression
response to activity
response to inactivity
negative regulation of myeloid dendritic cell activation
negative regulation of B cell proliferation
response to lipopolysaccharide
negative regulation of interferon-gamma production
negative regulation of interleukin-12 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
receptor biosynthetic process
response to insulin
response to carbon monoxide
response to drug
negative regulation of tumor necrosis factor biosynthetic process
defense response to bacterium
negative regulation of apoptotic process
negative regulation of growth of symbiont in host
negative regulation of nitric oxide biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
positive regulation of cytokine secretion
negative regulation of inflammatory response
negative regulation of membrane protein ectodomain proteolysis
positive regulation of sequence-specific DNA binding transcription factor activity
response to glucocorticoid
regulation of sensory perception of pain
branching involved in labyrinthine layer morphogenesis
cellular response to lipopolysaccharide
cellular response to estradiol stimulus
regulation of complement-dependent cytotoxicity
negative regulation of endothelial cell apoptotic process
