Invitrogen

IL-17A Polyclonal Antibody, PeproTech®

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IL-17A Antibody (500-P07-1MG) in WB

Western Blot: To detect Human IL-17A by Western Blot analysis IL-17A Polyclonal Antibody (Product # 500-P07-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P07-1MG

应用

建议稀释比

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免疫印迹 (WB)

0.1-0.2 µg/mL

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免疫组化 (IHC)

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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免疫染色 (IS)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human IL-17A

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2929172

产品详细信息

AA Sequence of recombinant protein: MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17A. Anti-Human IL-17A-specific antibody was purified by affinity chromatography employing an immobilized Human IL-17 matrix.

Sandwich ELISA: To detect Human IL-17A by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-17A (500-P07Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-17A.

Western Blot: To detect Human IL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Th17 cell-derived IL-17A promoted tumor progression via STAT3/NF-κB/Notch1 signaling in non-small cell lung cancer.

Oncoimmunology

Wang R,Yang L,Zhang C,Wang R,Zhang Z,He Q,Chen X,Zhang B,Qin Z,Wang L,Zhang Y

500-P07 was used in Immunohistochemistry immunofluorescence to investigate IL-17A driving of non-small cell lung carcinoma via signalling pathways.

Thu Feb 04 00:00:00 EST 2021

IL-17A, but not IL-17F, is indispensable for airway vascular remodeling induced by exaggerated Th17 cell responses in prolonged ovalbumin-challenged mice.

Journal of immunology (Baltimore, Md. : 1950)

Lu S,Li H,Gao R,Gao X,Xu F,Wang Q,Lu G,Xia D,Zhou J

Wed Apr 15 00:00:00 EDT 2015

The importance of testing anti-IL-17 antibodies from different suppliers.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Yapici Ü,Roelofs JJ,Florquin S

500-P07 was used in Immunohistochemistry to highlight the importance of testing anti-IL-17 antibodies from different suppliers.

Wed Feb 01 00:00:00 EST 2012

Neither IL-17A mRNA nor IL-17A protein are detectable in Langerhans cell histiocytosis lesions.

Molecular therapy : the journal of the American Society of Gene Therapy

Peters TL,McClain KL,Allen CE

500-P07 was used in Western Blotting to investigate the etiology of Langerhans cell histiocytosis (LCH) to demonstrate a failure to identify any cells in LCH lesions with interleukin-17 (IL-17A) gene expression.

Mon Aug 01 00:00:00 EDT 2011

Neither IL-17A mRNA nor IL-17A protein are detectable in Langerhans cell histiocytosis lesions.

Molecular therapy : the journal of the American Society of Gene Therapy

Peters TL,McClain KL,Allen CE

Mon Aug 01 00:00:00 EDT 2011

Neither IL-17A mRNA nor IL-17A protein are detectable in Langerhans cell histiocytosis lesions.

Molecular therapy : the journal of the American Society of Gene Therapy

Peters TL,McClain KL,Allen CE

Mon Aug 01 00:00:00 EDT 2011

Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.

Journal of immunology (Baltimore, Md. : 1950)

Brucklacher-Waldert V,Steinbach K,Lioznov M,Kolster M,Hölscher C,Tolosa E

Sun Nov 01 00:00:00 EDT 2009

生物信息学

蛋白别名: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin17A; R-IL-17-A

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基因别名: CTLA-8; CTLA8; IL-17; IL-17A; IL17; IL17A

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UniProt ID: (Human) Q16552

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Entrez Gene ID: (Human) 3605

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功能

cytokine activity cytokine receptor binding protein binding

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