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IL-2 Antibody (PA5-47217) in ICC/IF
Immunocytochemistry analysis of IL-2 in immersion fixed feline peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin. Samples were incubated in IL-2 polyclonal antibody (Product # PA5-47217) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
产品信息
PA5-47217
应用
建议稀释比
已发表文章
产品规格
种属反应
Cat
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant feline IL-2 Ala21-Thr154 (Cys146Ser)
偶联物
Unconjugated
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2608060
产品详细信息
In sandwich ELISAs, approximately 3% cross-reactivity with recombinant canine IL-2 is observed, approximately 1% cross reactivity with recombinant human IL-2 and recombinant porcine IL-2 is observed, and less than 0.2% cross-reactivity with recombinant mouse IL-2, recombinant equine IL-2, recombinant bovine IL-2, recombinant rat IL-2, and recombinant cotton rat IL-2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
靶标信息
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF
基因别名: IL2
UniProt ID: (Cat) Q07885
Entrez Gene ID: (Cat) 751114
