Invitrogen

IL-21 Polyclonal Antibody

高级验证

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

查看其他36个IL-21抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

IL-21 Antibody in Immunocytochemistry (ICC/IF)

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IL-21 Antibody (PA5-46962) in ICC/IF

Immunocytochemistry analysis of IL-21 in immersion fixed concanavalin A-stimulated mouse splenocytes. Samples were incubated in IL-21 polyclonal antibody (Product # PA5-46962) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained (green).

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IL-21 Antibody — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of IL-21 is shown by the decrease in mean RFU (relative fluorescent units - measure of proliferation) with increasing concentrations of IL-21 Polyclonal Antibody (PA5-46962). {Neu}

产品信息

PA5-46962

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

免疫细胞化学 (ICC/IF)

5-15 µg/mL

酶联免疫吸附实验 (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

中和实验 (Neu)

0.2-2 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived recombinant mouse IL-21 Pro25-Ser146

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2605560

产品详细信息

In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse (rm) IL-2, rmIL-4, rmIL-15, and recombinant human IL-21 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.30 EU per 1 µg of the antibody by the LAL method.

靶标信息

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

仅用于科研。不用于诊断过程。未经明确授权不得转售。