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IL-27 Antibody (MA5-23551) in Flow
Flow cytometry of IL-27 in RAW 264.7 mouse monocyte/macrophage cell line. Samples were incubated in IL-27 monoclonal antibody (Product # MA5-23551) or isotype control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.
产品信息
MA5-23551
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
234205
抗原
CHO-derived recombinant mouse IL-27 (aa 29-234 of p28, aa 19-228 of EBI-3)
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
纯化类型
Protein A/G
保存液
Buffered saline solution with BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2610324
产品详细信息
This antibody recognizes the p28 subunit either as part of a p28/EBI-3 heterodimer or as a free subunit.
靶标信息
IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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生物信息学
蛋白别名: Epstein-Barr virus-induced gene 3 protein homolog; IL-27 p28 subunit; IL-27 subunit alpha; IL-27 subunit beta; IL-27-A; IL-27B; IL27 p28; IL27-A; ILN; Interleukin; interleukin 30; Interleukin-27 subunit alpha; Interleukin-27 subunit beta; M-IL-27 (p28); MGC71873; p28
基因别名: EBI-3; Ebi3; IL-27; IL-27p28; Il27; Il27a; Il27b; Il30; p28
UniProt ID: (Mouse) O35228, (Mouse) Q8K3I6
Entrez Gene ID: (Mouse) 50498, (Mouse) 246779
