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IL-3 Antibody (500-P53-1MG) in WB
Western Blot: To detect Murine IL-3 by Western Blot analysis IL-3 Polyclonal Antibody (Product # 500-P53-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P53-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine IL-3
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930612
产品详细信息
AA Sequence of recombinant protein: MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-3. Anti-Murine IL-3-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-3 matrix.
Sandwich ELISA: To detect Murine IL-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-3 (500-P53Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-3.
Western Blot: To detect Murine IL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: BP/BPA; Colony stimulating factor multiple; Eo-CSF; Hematopoietic growth factor; IL-3; ILN; Interleukin; Interleukin 3 (colony stimulating factor; Interleukin-3; Interleukin3; Mast cell growth factor; MCGF; MGC79398; MGC79399; Multi CSF; Multilineage colony stimulating factor; multiple); Multipotential colony-stimulating factor; P cell stimulating factor; P-cell-stimulating factor; PCSA
基因别名: BPA; Csfmu; HCGF; Il-3; Il3; MCGF; PSF
UniProt ID: (Mouse) P01586
Entrez Gene ID: (Mouse) 16187
regulation of cell growth
response to hypoxia
positive regulation of protein phosphorylation
positive regulation of myeloid leukocyte differentiation
regulation of glycolytic process
immune response
positive regulation of cell proliferation
response to hormone
regulation of gene expression
negative regulation of autophagy
cytokine-mediated signaling pathway
hemopoiesis
negative regulation of mast cell apoptotic process
myeloid cell apoptotic process
regulation of protein dephosphorylation
negative regulation of apoptotic process
positive regulation of JNK cascade
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of mast cell proliferation
extrinsic apoptotic signaling pathway in absence of ligand
