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Invitrogen
IL-4 Polyclonal Antibody
高级验证
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
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IL-4 Antibody (PA5-47930) in Neu
Neutralization of IL-4 in TF-1 human erythroleukemic cell line. Samples were incubated in IL-4 polyclonal antibody (Product # PA5-47930) using a dilution of 50 ng/mL. Recombinant Canine IL-4 stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-4 is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-4 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 2-8 µg/mL.
产品信息
PA5-47930
产品规格
种属反应
Dog
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant canine IL-4 His25-His132
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2609990
产品详细信息
In direct ELISAs, approximately 40% cross-reactivity with recombinant human IL-4 is observed, 15% cross-reactivity with recombinant feline IL-4 and recombinant porcine IL-4 is observed and less than 1% cross-reactivity with recombinant cotton rat IL-4, recombinant mouse IL-4, and recombinant rat IL-4 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.01 EU per 1 µg of the antibody by the LAL method.
靶标信息
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B-cell stimulatory factor 1; BSF-1; cytokine; H-IL-4; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
基因别名: IL-4; IL4
UniProt ID: (Dog) O77762
Entrez Gene ID: (Dog) 403785
positive regulation of macroautophagy
positive regulation of B cell proliferation
positive regulation of T cell proliferation
B cell activation
regulation of phosphorylation
negative regulation of apoptotic process
positive regulation of MHC class II biosynthetic process
negative regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
regulation of immune response
