Invitrogen

IL-6 Polyclonal Antibody, PeproTech®

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IL-6 Antibody (500-P73-1MG) in ELISA

Sandwich ELISA: To detect Rat IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-6 Polyclonal Antibody (Product # 500-P73-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-6 Polyclonal Antibody, Biotin (Product # 500-P73BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-6.

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产品信息

500-P73-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

中和实验 (Neu)

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产品规格

种属反应

Rat

已发表种属

Rat

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Rat IL-6

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2930726

产品详细信息

AA Sequence of recombinant protein: MFPTSQVRRG DFTEDTTHNR PVYTTSQVGG LITYVLREIL EMRKELCNGN SDCMNSDDAL SENNLKLPEI QRNDGCFQTG YNQEICLLKI CSGLLEFRFY LEFVKNNLQD NKKDKARVIQ SNTETLVHIF KQEIKDSYKI VLPTPTSNAL LMEKLESQKE WLRTKTIQLI LKALEEFLKV TMRSTRQT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-6. Anti-Rat IL-6-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-6 matrix.

Sandwich ELISA: To detect Rat IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-6 (500-P73Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-6.

Western Blot: To detect Rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

仅用于科研。不用于诊断过程。未经明确授权不得转售。