Invitrogen

IL-6 Monoclonal Antibody (MP5-20F3), eFluor™ 450, eBioscience™

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IL-6 Antibody (48-7061-82) in Flow

Mouse thioglycolate-elicited peritoneal exudate cells were either unstimulated (left) or stimulated with LPS in the presence of Brefeldin A (Product # 00-4506-51) overnight (right). The cells were surface stained with Anti-Mouse F4/80 Antigen eFluor® 660 (Product # 50-4801-82) and intracellularly stained with 0.25 µg of Anti-Mouse IL-6 eFluor® 450 using the Intracellular Fixation & Perbeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), ... View More

IL-6 Antibody in Immunocytochemistry (ICC/IF)

IL-6 Antibody in Immunohistochemistry (IHC)

IL-6 Antibody in Immunohistochemistry, ELISA (IHC, ELISA)

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产品信息

48-7061-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看6篇已发表文章 6篇已发表文章

酶联免疫吸附实验 (ELISA)

查看6篇已发表文章 6篇已发表文章

中和实验 (Neu)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MP5-20F3

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2574103

产品详细信息

Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported: This MP5-20F3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MP5-20F3 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse thioglycolate-elicited peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.

Journal of virology

Jeong GU,Lee S,Kim DY,Lyu J,Yoon GY,Kim KD,Ku KB,Ko J,Kwon YC

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Flow Cytometry

Thu Jun 29 00:00:00 EDT 2023

A low-carbohydrate diet induces hepatic insulin resistance and metabolic associated fatty liver disease in mice.

Molecular metabolism

Long F,Bhatti MR,Kellenberger A,Sun W,Modica S,Höring M,Liebisch G,Krieger JP,Wolfrum C,Challa TD

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Neutralization

Wed Mar 01 00:00:00 EST 2023

Microbially-Induced Exosomes from Dendritic Cells Promote Paracrine Immune Senescence: Novel Mechanism of Bone Degenerative Disease in Mice.

Aging and disease

Elsayed R,Elashiry M,Liu Y,Morandini AC,El-Awady A,Elashiry MM,Hamrick M,Cutler CW

48-7061-82 was used in Flow cytometry/Cell sorting to conclude that immune senescence, compounded by advanced age, and accelerated by oral dysbiosis and its induced SASP exosomes, plays a pivotal role in the pathophysiology of experimental periodontitis.

Wed Feb 01 00:00:00 EST 2023

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Immunocytochemistry

Mon Feb 28 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Immunocytochemistry

Mon Feb 28 00:00:00 EST 2022

Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia.

The Journal of experimental medicine

Rupert JE,Narasimhan A,Jengelley DHA,Jiang Y,Liu J,Au E,Silverman LM,Sandusky G,Bonetto A,Cao S,Lu X,O'Connell TM,Liu Y,Koniaris LG,Zimmers TA

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Neutralization

Mon Jun 07 00:00:00 EDT 2021

Restoration of 5-methoxytryptophan protects against atherosclerotic chondrogenesis and calcification in ApoE^-/- mice fed high fat diet.

Journal of biomedical science

Lee GL,Liao TL,Wu JY,Wu KK,Kuo CC

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in ELISA

Mon Nov 08 00:00:00 EST 2021

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in ELISA

Fri Jul 02 00:00:00 EDT 2021

Betulinic Acid Ameliorates the Severity of Acute Pancreatitis via Inhibition of the NF-κB Signaling Pathway in Mice.

International journal of molecular sciences

Zhou Z,Choi JW,Shin JY,Kim DU,Kweon B,Oh H,Kim YC,Song HJ,Bae GS,Park SJ

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Immunocytochemistry

Sat Jun 26 00:00:00 EDT 2021

Spatial proteomics revealed a CX₃CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder.

Mucosal immunology

Bottek J,Soun C,Lill JK,Dixit A,Thiebes S,Beerlage AL,Horstmann M,Urbanek A,Heuer H,Uszkoreit J,Eisenacher M,Bracht T,Sitek B,Hoffmann F,Vijitha N,von Eggeling F,Engel DR

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Immunohistochemistry

Wed Jul 01 00:00:00 EDT 2020

Spatial proteomics revealed a CX₃CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder.

Mucosal immunology

Bottek J,Soun C,Lill JK,Dixit A,Thiebes S,Beerlage AL,Horstmann M,Urbanek A,Heuer H,Uszkoreit J,Eisenacher M,Bracht T,Sitek B,Hoffmann F,Vijitha N,von Eggeling F,Engel DR