Proteintech

INSIG2 Polyclonal Antibody

12 篇参考文献

查看其他4个INSIG2抗体

抗体检测数据 (7)

INSIG2 Antibody in Immunocytochemistry (ICC/IF)

INSIG2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

INSIG2 Antibody (24766-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 24766-1-AP (INSIG2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

INSIG2 Antibody in Immunocytochemistry (ICC/IF)

INSIG2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

INSIG2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

INSIG2 Antibody in Western Blot (WB)

INSIG2 Antibody in Western Blot (WB)

INSIG2 Antibody in Western Blot (WB)

INSIG2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

INSIG2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 24766-1-AP (INSIG2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

INSIG2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver tissue slide using 24766-1-AP ( INSIG2 Antibody) at dilution of 1:50 (under 40x lens).

INSIG2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver tissue slide using 24766-1-AP ( INSIG2 Antibody) at dilution of 1:50 (under 10x lens).

INSIG2 Antibody in Western Blot (WB) — Rat liver tissue were subjected to SDS PAGE followed by western blot with 24766-1-AP ( INSIG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

INSIG2 Antibody in Western Blot (WB) — SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

INSIG2 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

INSIG2 Antibody in Immunoprecipitation (IP) — IP result of anti-INSIG2 (IP:24766-1-AP, 4ug; Detection:24766-1-AP 1:500) with mouse liver tissue lysate 3600ug.

产品信息

24766-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看8篇已发表文章 8篇已发表文章

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200

-

免疫细胞化学 (ICC/IF)

1:10-1:100

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

0.5-4.0 µg

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

INSIG2 Fusion Protein Ag14072 (79-139 aa encoded by BC022475)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.4 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: TASAVIGLL YPCIDRHLGE PHKFKREWSS VMRCVAVFVG INHASAKVDF DNNIQLSLTL AA (79-139 aa encoded by BC022475)

靶标信息

INSIG2 is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi.The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Multi-Level Systems Biology Analysis of Aldrin's Metabolic Effects on Prostate Cancer Cells.

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Bedia C,Dalmau N,Nielsen LK,Tauler R,Marín de Mas I

Thu Mar 23 00:00:00 EDT 2023

Insulin-induced gene 2 expression is associated with cervical adenocarcinoma malignant behavior.

Molecular biology reports

Qian Y,Li X,Qiu J,Zhang R

Wed Feb 01 00:00:00 EST 2023

Insulin-induced gene 2 expression is associated with cervical adenocarcinoma malignant behavior.

Molecular biology reports

Qian Y,Li X,Qiu J,Zhang R

Wed Feb 01 00:00:00 EST 2023

In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion.

Nature communications

Vatandaslar H,Garzia A,Meyer C,Godbersen S,Brandt LTL,Griesbach E,Chao JA,Tuschl T,Stoffel M

Fri Jun 09 00:00:00 EDT 2023

Dickkopf-1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li X,Zheng T,Zhang Y,Zhao Y,Liu F,Dai S,Liu X,Zhang M

Tue Aug 01 00:00:00 EDT 2023

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Molecular metabolism

Zeng H,Qin H,Liao M,Zheng E,Luo X,Xiao A,Li Y,Chen L,Wei L,Zhao L,Ruan XZ,Yang P,Chen Y

Tue Mar 01 00:00:00 EST 2022

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Molecular metabolism

Zeng H,Qin H,Liao M,Zheng E,Luo X,Xiao A,Li Y,Chen L,Wei L,Zhao L,Ruan XZ,Yang P,Chen Y

Tue Mar 01 00:00:00 EST 2022

The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1.

International journal of biological sciences

Hu M,Han T,Pan Q,Ni D,Gao F,Wang L,Ren H,Zhang X,Jiao H,Wang Y,Dai D,Man Y,Tang W,Sun Y,Li W,Li J,Li G

Thu Jun 02 00:00:00 EDT 2022

The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1.

International journal of biological sciences

Hu M,Han T,Pan Q,Ni D,Gao F,Wang L,Ren H,Zhang X,Jiao H,Wang Y,Dai D,Man Y,Tang W,Sun Y,Li W,Li J,Li G

Thu Jun 02 00:00:00 EDT 2022

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Nature

Xu D,Wang Z,Xia Y,Shao F,Xia W,Wei Y,Li X,Qian X,Lee JH,Du L,Zheng Y,Lv G,Leu JS,Wang H,Xing D,Liang T,Hung MC,Lu Z

Wed Apr 01 00:00:00 EDT 2020

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Nature

Xu D,Wang Z,Xia Y,Shao F,Xia W,Wei Y,Li X,Qian X,Lee JH,Du L,Zheng Y,Lv G,Leu JS,Wang H,Xing D,Liang T,Hung MC,Lu Z

Wed Apr 01 00:00:00 EDT 2020

p38α/S1P/SREBP2 activation by the SAM-competitive EZH2 inhibitor GSK343 limits its anticancer activity but creates a druggable vulnerability in hepatocellular carcinoma.

American journal of cancer research

Yang PM,Hong YH,Hsu KC,Liu TP

Tue Sep 29 00:00:00 EDT 2020

生物信息学

蛋白别名: INSIG-2; INSIG-2 membrane protein; INSIG2 membrane protein; insulin induced protein 2; Insulin-induced gene 2 protein; MGC26273

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基因别名: 2900053I11Rik; C730043J18Rik; INSIG-2; INSIG2

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UniProt ID: (Human) Q9Y5U4, (Rat) Q80UA9, (Mouse) Q91WG1

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Entrez Gene ID: (Human) 51141, (Rat) 288985, (Mouse) 72999

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protein binding transcription factor binding

参与通路

triglyceride metabolic process cholesterol biosynthetic process negative regulation of steroid biosynthetic process response to insulin SREBP signaling pathway inner ear morphogenesis middle ear morphogenesis negative regulation of fatty acid biosynthetic process palate development cranial suture morphogenesis response to fatty acid response to sterol depletion cholesterol metabolic process sterol biosynthetic process response to lipid lipid metabolic process steroid metabolic process