Invitrogen

IP3 Receptor 2 Polyclonal Antibody

IP3 Receptor 2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

IP3 Receptor 2 Antibody (PA1-904) in ICC/IF

Immunofluorescence analysis of IP3 Receptor II was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IP3 Receptor II Rabbit Polyclonal Antibody (Product # PA1-904) at 2 µg/mL in 0.1% BSA and incubated for 3 hours ... View More

IP3 Receptor 2 Antibody in Immunohistochemistry (IHC)

IP3 Receptor 2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

产品信息

PA1-904

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（冰冻） (IHC (F))

1:100

免疫细胞化学 (ICC/IF)

1:100

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Amphibian, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: E(317) L N P D Y R D A Q N E G K T V R D(334).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2130127

产品详细信息

PA1-904 detects inositol 1,4,5-trisphosphate receptor type-II (IP3R-II) from rat and mouse tissues.

PA1-904 has been successfully used in immunohistochemistry, immunofluorescence and immunocytochemistry procedures. Immunohistochemical staining of IP3R-II in rat brain with PA1-904 results in staining of the hippocampus, corpus collosum, and cerebellum. This product fails to detect IP3R-II in Western blot procedures.

PA1-904 immunizing peptide corresponds to amino acid residues 317-334 from rat IP3R-II. PA1-904 immunizing peptide (Cat. # PEP-024) is available for use in neutralization and control experiments.

靶标信息

Inositol 1,4,5-trisphosphate (IP3) is a second messenger for many growth factors, hormones, and neuro-transmitters. Upon binding to IP3R, IP3 triggers the release of intracellular, luminal calcium to the cytosol. Functional IP3R is a homo- or heterotetramer of ~240 kDa glycoprotein subunits. IP3R protein is structurally and functionally related to one other important intracellular calcium-release channel, the ryanodine receptor. The similarity between the two receptors continues at the physiological level owing to a physical association each receptor can have with the immunophilin protein, FKBP12. Phosphorylation of the IP3R by PKC causes an increase in IP3-mediated calcium release. Concomitantly, the phosphatase activity of calcineurin is stimulated upon its association with the FKBP12-IP3R complex. Calcium release is reduced when the PKC target site on the IP3R is dephosphorylated by calcineurin resulting in calcium oscillations. Mammalian IP3R subunits are the product of three distinct genes that are widely expressed and differentially regulated. IP3R type I (IP3R-I) has been detected in heart, liver, kidney, ovary, and Purkinje neurons of the cerebellum. IP3R-II is found predominantly in the brain. IP3R-III is known to be expressed in pancreatic islets, kidney, and the gastrointestinal tract.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Glycosaminoglycan mimetics trigger IP3-dependent intracellular calcium release in myoblasts.

Matrix biology : journal of the International Society for Matrix Biology

Martelly I,Singabraya D,Vandebrouck A,Papy-Garcia D,Cognard C,Raymond G,Guillet-Deniau I,Courty J,Constantin B

PA1-904 was used in immunocytochemistry to investigate the effect of glycosaminoglycan mimetics on calcium signaling in myoblasts

Sat May 01 00:00:00 EDT 2010

Paclitaxel accelerates spontaneous calcium oscillations in cardiomyocytes by interacting with NCS-1 and the InsP3R.

Journal of molecular and cellular cardiology

Zhang K,Heidrich FM,DeGray B,Boehmerle W,Ehrlich BE

PA1-904 was used in western blot to investigate the effect of taxol on calcium signaling and calcium oscillation

Mon Nov 01 00:00:00 EDT 2010

Multiple receptor interactions trigger release of membrane and intracellular calcium stores critical for herpes simplex virus entry.

Molecular biology of the cell

Cheshenko N,Liu W,Satlin LM,Herold BC

PA1-904 was used in immunocytochemistry to study the role of calcium in herpes simplex viruses entry.

Wed Aug 01 00:00:00 EDT 2007

Mini-dystrophin expression down-regulates IP3-mediated calcium release events in resting dystrophin-deficient muscle cells.

The Journal of general physiology

Balghi H,Sebille S,Mondin L,Cantereau A,Constantin B,Raymond G,Cognard C

PA1-904 was used in immunocytochemistry to study how dystrophin affects IP3-mediaed calcium signaling pathway in resting myotubes

Tue Aug 01 00:00:00 EDT 2006

Mini-dystrophin expression down-regulates IP3-mediated calcium release events in resting dystrophin-deficient muscle cells.

The Journal of general physiology

Balghi H,Sebille S,Mondin L,Cantereau A,Constantin B,Raymond G,Cognard C

PA1-904 was used in immunocytochemistry to study how dystrophin affects IP3-mediaed calcium signaling pathway in resting myotubes

Tue Aug 01 00:00:00 EDT 2006

Mini-dystrophin expression down-regulates overactivation of G protein-mediated IP3 signaling pathway in dystrophin-deficient muscle cells.

The Journal of general physiology

Balghi H,Sebille S,Constantin B,Patri S,Thoreau V,Mondin L,Mok E,Kitzis A,Raymond G,Cognard C

PA1-904 was used in immunocytochemistry to demonstrate the existence of a potential relationship between mini-dystrophin and SR calcium release

Wed Feb 01 00:00:00 EST 2006

Mini-dystrophin expression down-regulates overactivation of G protein-mediated IP3 signaling pathway in dystrophin-deficient muscle cells.

The Journal of general physiology

Balghi H,Sebille S,Constantin B,Patri S,Thoreau V,Mondin L,Mok E,Kitzis A,Raymond G,Cognard C

PA1-904 was used in immunocytochemistry to demonstrate the existence of a potential relationship between mini-dystrophin and SR calcium release

Wed Feb 01 00:00:00 EST 2006

Immunolocalization of type 2 inositol 1,4,5-trisphosphate receptors in cardiac myocytes from newborn mice.

American journal of physiology. Cell physiology

García KD,Shah T,García J

PA1-904 was used in immunocytochemistry to investigate the localization of type 2 inositol 1,4,5-trisphosphate receptors (InsP (3) Rs) in cardiac muscle cells

Fri Oct 01 00:00:00 EDT 2004

C(a2+)-dependent glutamate release involves two classes of endoplasmic reticulum Ca(2+) stores in astrocytes.

Journal of neuroscience research

Hua X,Malarkey EB,Sunjara V,Rosenwald SE,Li WH,Parpura V

PA1-904 was used in immunocytochemistry to investigate the mechanism of glutamate release induced by calcium in astrocytes

Thu Apr 01 00:00:00 EST 2004

Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum).

The Journal of comparative neurology

Krizaj D,Liu X,Copenhagen DR

PA1-904 was used in immunohistochemistry to investigate how cytoplasmic free calcium in neurons from the salamander retina is regulated

Mon Aug 02 00:00:00 EDT 2004

ET-1 activates Ca2+ sparks in PASMC: local Ca2+ signaling between inositol trisphosphate and ryanodine receptors.

American journal of physiology. Lung cellular and molecular physiology

Zhang WM,Yip KP,Lin MJ,Shimoda LA,Li WH,Sham JS

PA1-904 was used in immunohistochemistry to suggest that ET-1 activation of calcium sparks is mediated via the ETA receptor-PLC-IP3 pathway

Mon Sep 01 00:00:00 EDT 2003

Sparks and puffs in oligodendrocyte progenitors: cross talk between ryanodine receptors and inositol trisphosphate receptors.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Haak LL,Song LS,Molinski TF,Pessah IN,Cheng H,Russell JT

PA1-904 was used in immunocytochemistry to investigate the interaction between ryanodine receptors and inositol trisphosphate receptors in oligodendrocyte progenitors.

Fri Jun 01 00:00:00 EDT 2001

Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction.

BMC neuroscience

Clapp TR,Stone LM,Margolskee RF,Kinnamon SC

PA1-904 was used in immunocytochemistry to study the role of type III IP3 receptor (IP3R3) in bitter taste transduction

Wed Apr 16 00:00:00 EDT 2003

生物信息学

蛋白别名: cilia and flagella associated protein 48; inositol 1,4,5-triphosphate receptor 2; inositol 1,4,5-triphosphate receptor 5; inositol 1,4,5-triphosphate receptor, type 2; Inositol 1,4,5-trisphosphate receptor type 2; Inositol 1,4,5-trisphosphate type V receptor; Inositol 1,4,5-trisphosphate-gated calcium channel ITPR2; inositol triphosphate receptor type 2; inositol trisphosphate receptor type 2; insP3R2; IP3 receptor; IP3 receptor isoform 2; IP3R 2; Type 2 inositol 1,4,5-trisphosphate receptor; Type 2 InsP3 receptor

查看更多收起

基因别名: AI649341; ANHD; CFAP48; InsP3R-2; InsP3R-5; INSP3R2; IP3R2; ITPR2; Itpr5

查看更多收起

UniProt ID: (Human) Q14571, (Mouse) Q9Z329, (Rat) P29995

查看更多收起

Entrez Gene ID: (Human) 3709, (Mouse) 16439, (Rat) 81678

查看更多收起

功能

inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity calcium ion transmembrane transporter activity phosphatidylinositol binding ion channel activity calcium channel activity calcium ion binding calcium-release channel activity inositol 1,4,5 trisphosphate binding ligand-gated ion channel

参与通路