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IRAK4 Antibody (700026) in ICC/IF
Immunofluorescent analysis of IRAK-4 in HeLa cells using an IRAK-4 recombinant rabbit monoclonal antibody (Product # 700026) at a dilution of 5 µg/mL followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained using Alexa Fluor 568 Phalloidin at a dilution of 1:200. Panels: Hoechst only (top left), AF488 signal only (top right) and composite image with Phalloidin (bottom left).
产品信息
700026
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
12H2L6
抗原
A peptide corresponding to amino acids 41-52 of Q9NWZ3.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532271
产品详细信息
This antibody is predicted to react with mouse, rat, primate, ovine, equine, porcine, bovine, canine and Xenopus based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: interleukin-1; Interleukin-1 receptor-associated kinase 4; IRAK-4; Renal carcinoma antigen NY-REN-64
基因别名: IPD1; IRAK-4; IRAK4; NY-REN-64; REN64
UniProt ID: (Human) Q9NWZ3
Entrez Gene ID: (Human) 51135
cytokine production
toll-like receptor signaling pathway
neutrophil mediated immunity
MyD88-dependent toll-like receptor signaling pathway
JNK cascade
cytokine-mediated signaling pathway
toll-like receptor 9 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of smooth muscle cell proliferation
neutrophil migration
