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IRE1a Antibody (BS-8680R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRE1a Polyclonal Antibody, Unconjugated (bs-8680R) at 1:400 overnight at 4°C, DAB staining.
产品信息
BS-8680R
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Pig,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide derived from human IRE1a, amino acids 252-260.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
内含物
0.02% ProClin 300
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; ERN1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1
基因别名: 9030414B18Rik; AI225830; C85377; ERN1; hIRE1p; IRE1; IRE1a; Ire1alpha; IRE1P; RGD1559716
UniProt ID: (Human) Q59EE2, (Mouse) Q9EQY0
Entrez Gene ID: (Human) 2081, (Pig) 100510978, (Mouse) 78943, (Rat) 498013
magnesium ion binding
endoribonuclease activity
protein serine/threonine kinase activity
protein binding
ATP binding
enzyme binding
Hsp70 protein binding
identical protein binding
protein homodimerization activity
ADP binding
Hsp90 protein binding
nucleotide binding
catalytic activity
endonuclease activity
ribonuclease activity
protein kinase activity
kinase activity
bidentate ribonuclease III activity
transferase activity
transferase activity, transferring phosphorus-containing groups
hydrolase activity
metal ion binding
endoribonuclease
endothelial cell proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
mRNA cleavage
mRNA catabolic process
protein phosphorylation
activation of signaling protein activity involved in unfolded protein response
cell cycle arrest
activation of JUN kinase activity
regulation of macroautophagy
positive regulation of RNA splicing
response to endoplasmic reticulum stress
cellular response to vascular endothelial growth factor stimulus
peptidyl-serine autophosphorylation
IRE1-mediated unfolded protein response
protein autophosphorylation
mRNA splicing, via endonucleolytic cleavage and ligation
mRNA endonucleolytic cleavage involved in unfolded protein response
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to glucose stimulus
RNA phosphodiester bond hydrolysis, endonucleolytic
positive regulation of endoplasmic reticulum unfolded protein response
insulin metabolic process
peptidyl-serine trans-autophosphorylation
mRNA processing
apoptotic process
response to unfolded protein
metabolic process
phosphorylation
endoplasmic reticulum unfolded protein response
mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response
RNA splicing
