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IRF2 Antibody (700226) in IHC (P)
Immunohistochemistry analysis of IRF2 showing staining in the nucleus of paraffin-embedded human T cell lymphoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, was... View More
产品信息
700226
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
B-80 H53L46
抗原
A recombinant protein corresponding to amino acids 121-270.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532300
产品详细信息
This antibody is predicted to react with bovine based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
IRF2 belongs to the interferon regulatory factor family. IRF2 regulates the transcription regulation important to cell proliferation as well as immune responses by binding to the upstream regulatory region of type I IFN and IFNinducible MHC class I genes (the interferon consensus sequence (ICS)) and repression of those genes. However, IRF2 also functions as a transcriptional activator of histone H4 by interactions with NF-kappa B.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp686F0244; Interferon regulatory factor 2; IRF-2; OTTHUMP00000218576; OTTHUMP00000218580
基因别名: 9830146E22Rik; AI646973; IRF-2; IRF2
UniProt ID: (Human) P14316, (Mouse) P23906
Entrez Gene ID: (Human) 3660, (Mouse) 16363, (Rat) 290749
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
regulatory region DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
blood coagulation
cell proliferation
positive regulation of transcription from RNA polymerase II promoter
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
transcription, DNA-templated
