Invitrogen

IRF7 Monoclonal Antibody (MNGPKL), PE, eBioscience™

图: 1 / 4

IRF7 Antibody (12-5829-82) in Flow

Mouse splenocytes were untreated (right, orange histogram) or treated with CpG ODN 2395 (right, purple histogram) for 5 hours. Using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, the cells were then intracellularly stained with Anti-Mouse CD317 (BST2, PDCA-1) FITC (Product # 11-3172-82), Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82), and 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (right, blue histogram) or 0.125 µg of Anti-Mouse IRF7 PE. Viable CD317+... View More

Product Image Gallery

产品信息

12-5829-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

MNGPKL

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572629

产品详细信息

Description: The monoclonal antibody MNGPKL reacts with mouse interferon regulatory factor 7 (IRF7), a 54 kDa transcription factor essential for induction of the antiviral innate immune response and IFN alpha production. B cells, plasmacytoid dendritic cells (pDC), and monocytes constitutively express IRF7 in the cytoplasm until activation by viral infection, double-stranded RNA or Toll-like receptor (TLR) signaling. CpG ODNs and single-stranded RNA, recognized by CD289 (TLR9) and TLR7, respectively, trigger IRF7 activation and upregulation. This activation promotes type I IFN response to disrupt pathogen and viral infection. These events causeIRF7 phosphorylation and translocation to the nucleus where it forms a homodimer to affect transcription.

Applications Reported: This MNGPKL antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MNGPKL antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.

仅用于科研。不用于诊断过程。未经明确授权不得转售。