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IRF9 Antibody (702322) in ICC/IF
For immunofluorescence analysis, Ramos cells were fixed and permeabilized for detection of endogenous IRF9 using Anti- IRF9 Recombinant Rabbit Monoclonal Antibody (Product # 702322, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of IRF9 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938)... View More
产品信息
702322
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
14H9L22
抗原
Peptides corresponding to Human IRF9 (aa 186-201, 369-384)
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633083
产品详细信息
This antibody is predicted to react with Monkey, Cat, Pig and Horse
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Transcription factor that plays an essential role in anti-viral immunity. It mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: gamma 48kDa; IFN alpha responsive transcription factor subunit; IFN-alpha-responsive transcription factor subunit; Interferon regulatory factor 9; Interferon-stimulated gene factor 3 gamma; interferon-stimulated transcription factor 3; interferon-stimulated transcription factor 3, gamma (48kD); interferon-stimulated transcription factor 3, gamma 48kDa; IRF-9; ISGF-3 gamma; ISGF3 p48 subunit; Transcriptional regulator ISGF3 subunit gamma
基因别名: IRF-9; IRF9; ISGF3; ISGF3G; p48
UniProt ID: (Human) Q00978
Entrez Gene ID: (Human) 10379
