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ISG15 Antibody (MA5-50361) in WB
Western Blot analysis of ISG15 using ISG15 Recombinant Monoclonal Antibody (Product # MA5-50361) in Untreated (–) and treated (+) A549 whole cell extracts (30 μg) using a dilution of 1:1,000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
产品信息
MA5-50361
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
HL2016
抗原
Recombinant fragment of human ISG15.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3093144
靶标信息
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hUCRP; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein, 15 kDa; IP17; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15
基因别名: G1P2; hUCRP; IFI15; IMD38; IP17; ISG15; UCRP
UniProt ID: (Human) P05161
Entrez Gene ID: (Human) 9636
modification-dependent protein catabolic process
translesion synthesis
negative regulation of protein ubiquitination
ISG15-protein conjugation
negative regulation of type I interferon production
regulation of interferon-gamma production
response to type I interferon
defense response to bacterium
negative regulation of viral genome replication
positive regulation of erythrocyte differentiation
defense response to virus
type I interferon signaling pathway
