OriGene

ISL1 Monoclonal Antibody (OTI2D8), TrueMAB™

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抗体检测数据 (1)

ISL1 Antibody in Western Blot (WB)

ISL1 Antibody in Western Blot (WB)

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ISL1 Antibody (CF807585) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISL1 (RC206893, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ISL1. (1. Positive lysates LY400801 (100 µg) and LC400801 (20 µg) can be purchased separately from OriGene.

ISL1 Antibody in Western Blot (WB)

Product Image Gallery

ISL1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISL1 (RC206893, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ISL1. (1. Positive lysates LY400801 (100 µg) and LC400801 (20 µg) can be purchased separately from OriGene.

产品信息

CF807585

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI2D8

抗原

Human recombinant protein fragment corresponding to amino acids 134-349 of human ISL1 produced in E.coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 8% trehalose

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

靶标信息

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Insulin gene enhancer protein ISL-1; ISL1 transcription factor, LIM/homeodomain; Islet-1

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基因别名: Isl-1; ISL1; ISLET1

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UniProt ID: (Human) P61371

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Entrez Gene ID: (Human) 3670

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity enhancer sequence-specific DNA binding chromatin binding protein binding zinc ion binding ligand-dependent nuclear receptor binding estrogen receptor binding bHLH transcription factor binding

参与通路

negative regulation of transcription from RNA polymerase II promoter neural crest cell migration secondary heart field specification outflow tract septum morphogenesis outflow tract morphogenesis endocardial cushion morphogenesis cardiac right ventricle morphogenesis regulation of secondary heart field cardioblast proliferation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of vascular endothelial growth factor production spinal cord motor neuron cell fate specification spinal cord motor neuron differentiation visceral motor neuron differentiation trigeminal nerve development pituitary gland development pancreas development axon regeneration retinal ganglion cell axon guidance positive regulation of insulin secretion positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-1 alpha production positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production negative regulation of intracellular estrogen receptor signaling pathway positive regulation of histone acetylation positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of DNA binding negative regulation of neuron apoptotic process negative regulation of neuron differentiation positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter neuron fate specification mesenchymal cell differentiation sensory system development peripheral nervous system neuron axonogenesis negative regulation of inflammatory response ventricular cardiac muscle tissue morphogenesis pharyngeal system development cardiac muscle cell myoblast differentiation innervation atrial septum morphogenesis cardiac cell fate determination cellular response to glucocorticoid stimulus positive regulation of granulocyte colony-stimulating factor production negative regulation of protein homodimerization activity negative regulation of canonical Wnt signaling pathway positive regulation of macrophage colony-stimulating factor production