Invitrogen

ITGB5 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

查看其他16个ITGB5 (Integrin beta 5)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (2)

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ITGB5 Antibody (PA5-49745) in WB

Western blot was performed using Anti-ITGB5 Polyclonal Antibody (Product # PA5-49745) and a 88 kDa band corresponding to ITGB5 was observed across cell lines tested and increased upon TGF beta treatment, along with an uncharacterized band (*) ~ 70 kDa in tissues tested. Membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with TGF beta (2 ng/mL for 24 Hours) (Lane 2), A549 (Lane 3) and tissue extracts (30 µg lysate) of Mouse Kidney (Lane 4) and Mouse Brain (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-... View More

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ITGB5 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with ITGB5 siRNA and decrease in signal intensity was observed in western blot application using Anti- ITGB5 Polyclonal Antibody (Product # PA5-49745). {KD}

产品信息

PA5-49745

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthesized peptide derived from human Integrin beta5.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2635198

产品详细信息

The antibody detects endogenous levels of total Integrin beta 5 protein.

靶标信息

Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around 22 different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in-groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。