Invitrogen

ITGB8 Monoclonal Antibody (CL7290)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他11个ITGB8 (Integrin beta 8)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

ITGB8 Antibody in Immunocytochemistry (ICC/IF)

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ITGB8 Antibody (MA5-31448) in ICC/IF

Immunofluorescence analysis of Integrin beta-8 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ITGB8 Monoclonal Antibody (CL7290) (Product # MA5-31448) at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa... View More

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ITGB8 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. A-431 cells were transfected with Integrin beta-8 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-ITGB8 Monoclonal Antibody (CL7290) (Product # MA5-31448). {KD}

产品信息

MA5-31448

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

- -

免疫细胞化学 (ICC/IF)

2-10 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

CL7290

抗原

Recombinant protein corresponding to Human ITGB8

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2787084

产品详细信息

Immunogen sequence: AQHCVNSKGQ VCSGRGTCVC GRCECTDPRS IGRFCEHCPT CYTACKENWN CMQCLHPHNL SQAILDQCKT SCALMEQQHY

靶标信息

ITGB8 (Integrin beta 8) is a member of the integrin beta chain family and is a single-pass type I membrane protein with a VWFA domain and four cysteine-rich repeats. This protein noncovalently binds to an alpha subunit to form a heterodimeric integrin complex. In general, integrin complexes mediate cell-cell and cell-extracellular matrix interactions and this complex plays a role in human airway epithelial proliferation. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

仅用于科研。不用于诊断过程。未经明确授权不得转售。