Product References
Vaccination against microbiota motility protects mice from the detrimental impact of dietary emulsifier consumption.
PLoS biology
Kordahi MC,Delaroque C,Bredèche MF,Gewirtz AT,Chassaing B
12-4204-82 was used in Immunocytochemistry to establish that eliciting mucosal immune responses to pathobiont surface components, including flagellin, may be a means of combatting the array of inflammatory diseases that are promoted by emulsifiers and perhaps other modern microbiota stressors.
Fri Sep 01 00:00:00 UTC 2023
Rad52 mediates class-switch DNA recombination to IgD.
Nature communications
Xu Y,Zhou H,Post G,Zan H,Casali P
12-4204-82 was used in Flow Cytometry to show that Rad52 mediates IgD CSR through microhomology-mediated A-EJ in concert with Zfp318 downregulation.
Mon Feb 21 00:00:00 UTC 2022
Rad52 mediates class-switch DNA recombination to IgD.
Nature communications
Xu Y,Zhou H,Post G,Zan H,Casali P
12-4204-82 was used in Flow Cytometry to show that Rad52 mediates IgD CSR through microhomology-mediated A-EJ in concert with Zfp318 downregulation.
Mon Feb 21 00:00:00 UTC 2022
SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination.
Nature communications
Vincendeau E,Wei W,Zhang X,Planchais C,Yu W,Lenden-Hasse H,Cokelaer T,Pipoli da Fonseca J,Mouquet H,Adams DJ,Alt FW,Jackson SP,Balmus G,Lescale C,Deriano L
12-4204-82 was used in Flow cytometry/Cell sorting to suggest that 53BP1 promotes V(D)J recombination and CSR through two distinct mechanisms: SHLD-independent synapsis of V(D)J segments and switch regions within chromatin, and SHLD-dependent protection of AID-DSB ends against resection.
Tue Jun 28 00:00:00 UTC 2022
Interspecies commensal interactions have nonlinear impacts on host immunity.
Cell host & microbe
Rice TA,Bielecka AA,Nguyen MT,Rosen CE,Song D,Sonnert ND,Yang Y,Cao Y,Khetrapal V,Catanzaro JR,Martin AL,Rashed SA,Leopold SR,Hao L,Yu X,van Dijk D,Ring AM,Flavell RA,de Zoete MR,Palm NW
12-4204-82 was used in Flow Cytometry to identify a pairwise reciprocal interaction between human gut bacteria that dictates divergent immunological outcomes.
Wed Jul 13 00:00:00 UTC 2022
Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity.
Nature immunology
Prados A,Onder L,Cheng HW,Mörbe U,Lütge M,Gil-Cruz C,Perez-Shibayama C,Koliaraki V,Ludewig B,Kollias G
Published figure using IgA monoclonal antibody (Product # 12-4204-82) in Flow Cytometry
Thu Apr 01 00:00:00 UTC 2021
Functional human IgA targets a conserved site on malaria sporozoites.
Science translational medicine
Tan J,Cho H,Pholcharee T,Pereira LS,Doumbo S,Doumtabe D,Flynn BJ,Schön A,Kanatani S,Aylor SO,Oyen D,Vistein R,Wang L,Dillon M,Skinner J,Peterson M,Li S,Idris AH,Molina-Cruz A,Zhao M,Olano LR,Lee PJ,Roth A,Sinnis P,Barillas-Mury C,Kayentao K,Ongoiba A,Francica JR,Traore B,Wilson IA,Seder RA,Crompton PD
12-4204-82 was used in Flow Cytometry to investigate the circulating IgA response in humans to P. falciparum sporozoites that are injected into the skin by mosquitoes and migrate to the liver via the bloodstream to initiate malaria infection.
Wed Jun 23 00:00:00 UTC 2021
Functional human IgA targets a conserved site on malaria sporozoites.
Science translational medicine
Tan J,Cho H,Pholcharee T,Pereira LS,Doumbo S,Doumtabe D,Flynn BJ,Schön A,Kanatani S,Aylor SO,Oyen D,Vistein R,Wang L,Dillon M,Skinner J,Peterson M,Li S,Idris AH,Molina-Cruz A,Zhao M,Olano LR,Lee PJ,Roth A,Sinnis P,Barillas-Mury C,Kayentao K,Ongoiba A,Francica JR,Traore B,Wilson IA,Seder RA,Crompton PD
12-4204-82 was used in Flow Cytometry to investigate the circulating IgA response in humans to P. falciparum sporozoites that are injected into the skin by mosquitoes and migrate to the liver via the bloodstream to initiate malaria infection.
Wed Jun 23 00:00:00 UTC 2021
ATR kinase activity promotes antibody class switch recombination in B cells through cell cycle regulation without suppressing DSB resection and microhomology usage.
Journal of leukocyte biology
Sun X,Liu M,Bai J,Xu J,Zhu C,Dong J,Chen C
Published figure using IgA monoclonal antibody (Product # 12-4204-82) in Flow Cytometry
Wed Dec 01 00:00:00 UTC 2021
TNFRSF13B polymorphisms counter microbial adaptation to enteric IgA.
JCI insight
Platt JL,de Mattos Barbosa MG,Huynh D,Lefferts AR,Katta J,Kharas C,Freddolino P,Bassis CM,Wobus C,Geha R,Bram R,Nunez G,Kamada N,Cascalho M
12-4204-82 was used in Flow Cytometry to show that C. rodentium and most likely other enteric organisms appropriated binding of otherwise protective antibodies to signal induction of the virulence program.
Thu Jul 22 00:00:00 UTC 2021
TNFRSF13B polymorphisms counter microbial adaptation to enteric IgA.
JCI insight
Platt JL,de Mattos Barbosa MG,Huynh D,Lefferts AR,Katta J,Kharas C,Freddolino P,Bassis CM,Wobus C,Geha R,Bram R,Nunez G,Kamada N,Cascalho M
12-4204-82 was used in Flow Cytometry to show that C. rodentium and most likely other enteric organisms appropriated binding of otherwise protective antibodies to signal induction of the virulence program.
Thu Jul 22 00:00:00 UTC 2021
Glycan-based shaping of the microbiota during primate evolution.
eLife
Singh S,Bastos-Amador P,Thompson JA,Truglio M,Yilmaz B,Cardoso S,Sobral D,Soares MP
Published figure using IgA monoclonal antibody (Product # 12-4204-82) in Flow Cytometry
Wed May 19 00:00:00 UTC 2021
Glycan-based shaping of the microbiota during primate evolution.
eLife
Singh S,Bastos-Amador P,Thompson JA,Truglio M,Yilmaz B,Cardoso S,Sobral D,Soares MP
Published figure using IgA monoclonal antibody (Product # 12-4204-82) in Flow Cytometry
Wed May 19 00:00:00 UTC 2021
Gut-Innervating Nociceptor Neurons Regulate Peyer's Patch Microfold Cells and SFB Levels to Mediate Salmonella Host Defense.
Cell
Lai NY,Musser MA,Pinho-Ribeiro FA,Baral P,Jacobson A,Ma P,Potts DE,Chen Z,Paik D,Soualhi S,Yan Y,Misra A,Goldstein K,Lagomarsino VN,Nordstrom A,Sivanathan KN,Wallrapp A,Kuchroo VK,Nowarski R,Starnbach MN,Shi H,Surana NK,An D,Wu C,Huh JR,Rao M,Chiu IM
12-4204-82 was used in Flow Cytometry to reveal a major role for nociceptor neurons in sensing and defending against enteric pathogens.
Thu Jan 09 00:00:00 UTC 2020
A Genetic Map of the Response to DNA Damage in Human Cells.
Cell
Olivieri M,Cho T,Álvarez-Quilón A,Li K,Schellenberg MJ,Zimmermann M,Hustedt N,Rossi SE,Adam S,Melo H,Heijink AM,Sastre-Moreno G,Moatti N,Szilard RK,McEwan A,Ling AK,Serrano-Benitez A,Ubhi T,Feng S,Pawling J,Delgado-Sainz I,Ferguson MW,Dennis JW,Brown GW,Cortés-Ledesma F,Williams RS,Martin A,Xu D,Durocher D
12-4204-82 was used in Flow Cytometry to provide a rich resource to study this fundamental cellular system and has implications for the development and use of genotoxic agents in cancer therapy.
Thu Jul 23 00:00:00 UTC 2020
Commensal Bacteria Modulate Immunoglobulin A Binding in Response to Host Nutrition.
Cell host & microbe
Huus KE,Bauer KC,Brown EM,Bozorgmehr T,Woodward SE,Serapio-Palacios A,Boutin RCT,Petersen C,Finlay BB
12-4204-82 was used in Flow Cytometry to study how host nutrition affects immune-microbe interactions using a mouse model.
Wed Jun 10 00:00:00 UTC 2020
Fecal IgA Levels Are Determined by Strain-Level Differences in Bacteroides ovatus and Are Modifiable by Gut Microbiota Manipulation.
Cell host & microbe
Yang C,Mogno I,Contijoch EJ,Borgerding JN,Aggarwala V,Li Z,Siu S,Grasset EK,Helmus DS,Dubinsky MC,Mehandru S,Cerutti A,Faith JJ
12-4204 was used in Flow cytometry/Cell sorting to assess the IgA-inducing capacity of a diverse set of human gut microbial strains by monocolonizing mice with each strain.
Wed Mar 11 00:00:00 UTC 2020
B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches.
Cell reports
Biram A,Winter E,Denton AE,Zaretsky I,Dassa B,Bemark M,Linterman MA,Yaari G,Shulman Z
12-4204-82 was used in Flow cytometry/Cell sorting to demonstrate that T cell-derived help to B cells in PPs includes SAP-dependent and SAP-independent functions.
Tue Feb 11 00:00:00 UTC 2020
REV7 is required for processing AID initiated DNA lesions in activated B cells.
Nature communications
Yang D,Sun Y,Chen J,Zhang Y,Fan S,Huang M,Xie X,Cai Y,Shang Y,Gui T,Sun L,Hu J,Dong J,Yeap LS,Wang X,Xiao W,Meng FL
12-4204 was used in Flow cytometry/Cell sorting to identify that REV7-depedent TLS across UNG-processed apurinic/apyrimidinic sites is required for cell survival upon AID/APOBEC deamination.
Thu Jun 04 00:00:00 UTC 2020
Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity.
Immunity
Martínez-López M,Iborra S,Conde-Garrosa R,Mastrangelo A,Danne C,Mann ER,Reid DM,Gaboriau-Routhiau V,Chaparro M,Lorenzo MP,Minnerup L,Saz-Leal P,Slack E,Kemp B,Gisbert JP,Dzionek A,Robinson MJ,Rupérez FJ,Cerf-Bensussan N,Brown GD,Bernardo D,LeibundGut-Landmann S,Sancho D
12-4204-82 was used in Flow Cytometry to conclude that sensing of commensals by Mincle and Syk signaling in CD11c+ cells reinforces intestinal immune barrier and promotes host-microbiota mutualism, preventing systemic inflammation.
Tue Feb 19 00:00:00 UTC 2019
Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity.
Immunity
Martínez-López M,Iborra S,Conde-Garrosa R,Mastrangelo A,Danne C,Mann ER,Reid DM,Gaboriau-Routhiau V,Chaparro M,Lorenzo MP,Minnerup L,Saz-Leal P,Slack E,Kemp B,Gisbert JP,Dzionek A,Robinson MJ,Rupérez FJ,Cerf-Bensussan N,Brown GD,Bernardo D,LeibundGut-Landmann S,Sancho D
12-4204-82 was used in Flow Cytometry to conclude that sensing of commensals by Mincle and Syk signaling in CD11c+ cells reinforces intestinal immune barrier and promotes host-microbiota mutualism, preventing systemic inflammation.
Tue Feb 19 00:00:00 UTC 2019
Intestinal Permeability and IgA Provoke Immune Vasculitis Linked to Cardiovascular Inflammation.
Immunity
Noval Rivas M,Wakita D,Franklin MK,Carvalho TT,Abolhesn A,Gomez AC,Fishbein MC,Chen S,Lehman TJ,Sato K,Shibuya A,Fasano A,Kiyono H,Abe M,Tatsumoto N,Yamashita M,Crother TR,Shimada K,Arditi M
12-4204-82 was used in Flow Cytometry to investigate intestinal barrier function in vasculitis and observe evidence of intestinal permeability and elevated circulating secretory immunoglobulin A (sIgA) in patients with Kawaski disease, as well as elevated sIgA and IgA deposition in vascular tissues in a mouse model of vasculitis.
Tue Sep 17 00:00:00 UTC 2019
Restriction of AID activity and somatic hypermutation by PARP-1.
Nucleic acids research
Tepper S,Mortusewicz O,Członka E,Bello A,Schmidt A,Jeschke J,Fischbach A,Pfeil I,Petersen-Mahrt SK,Mangerich A,Helleday T,Leonhardt H,Jungnickel B
12-4204-82 was used in Flow Cytometry to show that poly(ADP-ribose) polymerase-1 (PARP-1) is a key factor restricting AID activity during somatic hypermutation.
Thu Aug 22 00:00:00 UTC 2019
Antigen-presenting ILC3 regulate T cell-dependent IgA responses to colonic mucosal bacteria.
The Journal of experimental medicine
Melo-Gonzalez F,Kammoun H,Evren E,Dutton EE,Papadopoulou M,Bradford BM,Tanes C,Fardus-Reid F,Swann JR,Bittinger K,Mabbott NA,Vallance BA,Willinger T,Withers DR,Hepworth MR
12-4204-82 was used in Flow Cytometry to study the mechanisms that modulate the magnitude and quality of T cell-dependent IgA responses.
Mon Apr 01 00:00:00 UTC 2019
CHK1 dosage in germinal center B cells controls humoral immunity.
Cell death and differentiation
Schoeler K,Jakic B,Heppke J,Soratroi C,Aufschnaiter A,Hermann-Kleiter N,Villunger A,Labi V
Published figure using IgA monoclonal antibody (Product # 12-4204-82) in Flow Cytometry
Sun Dec 01 00:00:00 UTC 2019
Intestinal non-canonical NFκB signaling shapes the local and systemic immune response.
Nature communications
Ramakrishnan SK,Zhang H,Ma X,Jung I,Schwartz AJ,Triner D,Devenport SN,Das NK,Xue X,Zeng MY,Hu Y,Mortensen RM,Greenson JK,Cascalho M,Wobus CE,Colacino JA,Nunez G,Rui L,Shah YM
12-4204 was used in Magnetic cell separation to determine the role of Microfold cells in systemic inflammatory responses.
Fri Feb 08 00:00:00 UTC 2019
Intestinal non-canonical NFκB signaling shapes the local and systemic immune response.
Nature communications
Ramakrishnan SK,Zhang H,Ma X,Jung I,Schwartz AJ,Triner D,Devenport SN,Das NK,Xue X,Zeng MY,Hu Y,Mortensen RM,Greenson JK,Cascalho M,Wobus CE,Colacino JA,Nunez G,Rui L,Shah YM
12-4204 was used in Magnetic cell separation to determine the role of Microfold cells in systemic inflammatory responses.
Fri Feb 08 00:00:00 UTC 2019
Flagellin-elicited adaptive immunity suppresses flagellated microbiota and vaccinates against chronic inflammatory diseases.
Nature communications
Tran HQ,Ley RE,Gewirtz AT,Chassaing B
Published figure using IgA monoclonal antibody (Product # 12-4204-82) in Flow Cytometry
Wed Dec 11 00:00:00 UTC 2019
Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
Cellular and molecular gastroenterology and hepatology
Kiyohara H,Sujino T,Teratani T,Miyamoto K,Arai MM,Nomura E,Harada Y,Aoki R,Koda Y,Mikami Y,Mizuno S,Naganuma M,Hisamatsu T,Kanai T
12-4204 was used in Flow cytometry/Cell sorting to show that skin inflammation may contribute to pathogenic conditions in the gut via immunologic and microbiological changes.
Mon Apr 29 00:00:00 UTC 2019
Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells.
Cellular and molecular gastroenterology and hepatology
Kiyohara H,Sujino T,Teratani T,Miyamoto K,Arai MM,Nomura E,Harada Y,Aoki R,Koda Y,Mikami Y,Mizuno S,Naganuma M,Hisamatsu T,Kanai T
12-4204 was used in Flow cytometry/Cell sorting to show that skin inflammation may contribute to pathogenic conditions in the gut via immunologic and microbiological changes.
Mon Apr 29 00:00:00 UTC 2019
Vaccine-induced Th17 cells are established as resident memory cells in the lung and promote local IgA responses.
Mucosal immunology
Christensen D,Mortensen R,Rosenkrands I,Dietrich J,Andersen P
12-4204 was used in Flow cytometry/Cell sorting to demonstrate that subcutaneous priming with a recombinant antigen in a T helper (Th)17-inducing adjuvant followed by airway boosting promotes high and sustained levels of IgA in the lungs.
Sun Jan 01 00:00:00 UTC 2017
Allograft inflammatory factor 1 is a regulator of transcytosis in M cells.
Nature communications
Kishikawa S,Sato S,Kaneto S,Uchino S,Kohsaka S,Nakamura S,Kiyono H
Wed Feb 22 00:00:00 UTC 2017
Molecular definition of multiple sites of antibody inhibition of malaria transmission-blocking vaccine antigen Pfs25.
Nature communications
Scally SW,McLeod B,Bosch A,Miura K,Liang Q,Carroll S,Reponen S,Nguyen N,Giladi E,Rämisch S,Yusibov V,Bradley A,Lemiale F,Schief WR,Emerling D,Kellam P,King CR,Julien JP
12-4204 was used in Flow cytometry/Cell sorting to inform on the disposition of Pfs25 on the surface of ookinetes and provide the structural blueprints to design next-generation immunogens.
Thu Nov 16 00:00:00 UTC 2017
CD40-signalling abrogates induction of RORγt+ Treg cells by intestinal CD103+ DCs and causes fatal colitis.
Nature communications
Barthels C,Ogrinc A,Steyer V,Meier S,Simon F,Wimmer M,Blutke A,Straub T,Zimber-Strobl U,Lutgens E,Marconi P,Ohnmacht C,Garzetti D,Stecher B,Brocker T
12-4204 was used in Flow cytometry/Cell sorting to demonstrate a CD40-dependent mechanism capable of abrogating iTreg cell induction by dendritic cells and suggest that the CD40L/CD40-signalling axis might be able to intervene in the generation of new iTreg cells in order to counter-regulate immune suppression to enhance immunity.
Thu Mar 09 00:00:00 UTC 2017
Artery Tertiary Lymphoid Organs Control Multilayered Territorialized Atherosclerosis B-Cell Responses in Aged ApoE-/- Mice.
Arteriosclerosis, thrombosis, and vascular biology
Srikakulapu P,Hu D,Yin C,Mohanta SK,Bontha SV,Peng L,Beer M,Weber C,McNamara CA,Grassia G,Maffia P,Manz RA,Habenicht AJ
12-4204 was used in Flow cytometry/Cell sorting to study how artery tertiary lymphoid organs can orchestrate B-cell responses in the diseased aorta.
Wed Jun 01 00:00:00 UTC 2016
Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.
Nature communications
Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY
Published figure using IgA monoclonal antibody (Product # 12-4204-82) in Flow Cytometry
Tue Sep 06 00:00:00 UTC 2016
Checkpoint kinase 2 is required for efficient immunoglobulin diversification.
Cell cycle (Georgetown, Tex.)
Davari K,Frankenberger S,Schmidt A,Tomi NS,Jungnickel B
12-4204 was used in Flow cytometry/Cell sorting to investigate the role of checkpoint kinase 2 in immunoglobulin diversification through regulation of Ig hypermutation, class switching and gene conversion.
Thu Sep 10 00:00:00 UTC 2015
R-phycoerythrin-conjugated antibodies are inappropriate for intracellular staining of murine plasma cells.
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Kim MS,Kim TS
Published figure using IgA monoclonal antibody (Product # 12-4204-82) in Flow Cytometry
Wed May 01 00:00:00 UTC 2013
Microbe-dependent CD11b+ IgA+ plasma cells mediate robust early-phase intestinal IgA responses in mice.
Nature communications
Kunisawa J,Gohda M,Hashimoto E,Ishikawa I,Higuchi M,Suzuki Y,Goto Y,Panea C,Ivanov II,Sumiya R,Aayam L,Wake T,Tajiri S,Kurashima Y,Shikata S,Akira S,Takeda K,Kiyono H
12-4204 was used in Flow cytometry/Cell sorting to reveal the functional diversity of IgA(+) plasma cells in the murine intestine.
Tue Nov 26 00:00:00 UTC 2013
