Invitrogen

JAK2 Recombinant Rabbit Monoclonal Antibody (18H11L8)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2个已发表图片

3 篇参考文献

查看其他41个JAK2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

JAK2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

JAK2 Antibody (702434) in ICC/IF

For immunofluorescence analysis, MG-132 (20 uM, 1 h) and IFN gamma (100 ng, 15 min) treated HT-29 cells were fixed and permeabilized for detection of endogenous Jak2 using Anti-Jak2 Recombinant Rabbit Monoclonal Antibody (Product # 702434, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Jak2 protein (green), Panel b) is stained for nuclei (blue) using Sl... View More

Product Image Gallery

JAK2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot of JAK2 using Anti-JAK2 Recombinant Rabbit Monoclonal Antibody (Product # 702434), shows increased expression of JAK2 in HT-29 and HEL 92.1.7 upon treatment with MG132 followed by IFN Gamma. {TM}

产品信息

702434

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

18H11L8

抗原

Peptide corresponding to Human JAK2 (aa 1004-1012)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2633091

产品详细信息

This antibody is predicted to react with Monkey, Rat, Sheep and Bovine.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Janus kinase 2 (JAK2) is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways and is mutated in polycythemia vera. This kinase contains the catalytic domain (JH1) of JAK2. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex. Mutations affecting JAK2 can cause Budd-Chiari syndrome, Polycythemia vera, Thrombocythemia 3, myelofibrosis, or Acute myelogenous leukemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Discovery of a novel potentially transforming somatic mutation in CSF2RB gene in breast cancer.

Cancer medicine

Rashid M,Ali R,Almuzzaini B,Song H,AlHallaj A,Abdulkarim AA,Mohamed Baz O,Al Zahrani H,Mustafa Sabeena M,Alharbi W,Hussein M,Boudjelal M

702434 was used in Western Blotting to report the discovery of a mutation in the CSF2RB gene of a breast cancer patient and the cell line KAIMRC1.

Mon Nov 01 00:00:00 EDT 2021

Discovery of a novel potentially transforming somatic mutation in CSF2RB gene in breast cancer.

Cancer medicine

Rashid M,Ali R,Almuzzaini B,Song H,AlHallaj A,Abdulkarim AA,Mohamed Baz O,Al Zahrani H,Mustafa Sabeena M,Alharbi W,Hussein M,Boudjelal M

702434 was used in Western Blotting to report the discovery of a mutation in the CSF2RB gene of a breast cancer patient and the cell line KAIMRC1.

Mon Nov 01 00:00:00 EDT 2021

Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2.

Cells

Ali R,Al Zahrani H,Barhoumi T,Alhallaj A,Mashhour A,Alshammari MA,Alshawakir YA,Baz O,Alanazi AH,Khan AL,Al Nikhli H,Al Balwi MA,Al Riyees L,Boudjelal M

702434 was used in Western Blot to report the establishment of a naturally immortalized highly tumorigenic and triple-negative breast cancer cell line, KAIMRC2.

Mon May 24 00:00:00 EDT 2021

Role and mechanism of nursing cooperation and tetramethylpyrazine application in post-operative pain in patients undergoing total knee arthroplasty.

Experimental and therapeutic medicine

Liu C,Liu R,Tang M,Yang X,Gong X

702434 was used in Western Blotting to investigate the effect of nursing cooperation on the post-operative complication of pain following total knee arthroplasty and to explore the effects of tetramethylpyrazine application on post-TKA pain.