Invitrogen

K-Ras Monoclonal Antibody (9.13)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6个已发表图片

7 篇参考文献

查看其他24个K-Ras抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

K-Ras Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

K-Ras Antibody (415700) in ICC/IF

Immunofluorescence analysis of K-Ras was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with K-Ras (9.13) Mouse Monoclonal Antibody (Product # 415700) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a ... View More

K-Ras Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

K-Ras Antibody — Figure 2 miR-200c inhibits K-ras protein expression without affecting KRAS mRNA levels A) Luciferase reporter assay with different breast cancer cell lines. The renilla luciferase reporter containing the 3'UTR of KRAS including the predicted target site of miR-200c (RLuc) or the firefy luciferase control plasmid pGL3 (FLuc) were transfected into the indicated cell lines. Renilla reporter luciferase activity was normalized to the activity of the firefy control as ratio (RLuc/FLuc). Values are stated as mean +- SEM (n=5). B) Luciferase reporter assay upon miR-200c modulation. MDA-MB-436 cells were transfected with either pre-miR-200c (pre) or scrambled pre-miR-control (ctr). BT-474 cells were transfected with either miR-200c inhibitor (inh) or scrambled control inhibitor (ctr). Relative luciferase activities (RLuc/FLuc) are depicted in the graph. Values are stated as mean +- SEM (n=5). For statistical analysis a student's t-test was performed (**p<0.01; ***p<0.001). C) miR-200c inhibition in the miR-200c high cell lines BT-474 and MCF-7. Indicated cell lines were transfected with either miR-200c inhibitor (inh) or scrambled control inhibitor (ctr) and at 72h post transfection subjected to Western blot analysis (upper panel) or quantitative RT-PCR (lower panel). Values are stated as mean +- SD (n=3). D) miR-200c overexpression in the miR-200c low cell lines MDA-MB-231 and MDA-MB-436. Indicated cell lines were transfected with either pre-miR-200c (pre) or scrambled pre-miR-contro {KD}

产品信息

415700

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

1-2 µg/mL

免疫沉淀 (IP)

5 µg

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

9.13

抗原

Immunogen corresponds to aa 170-180 from P01116-1 (isoform 2A) of human K-Ras.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2532192

产品详细信息

This antibody is specific for human K-Ras (K-Ras2, Ki-Ras, c-K-ras, GTPase KRas) protein (accession # NP_004976.2, P01116), which is 100% homologous with mouse, 95% with rat, and 94% with bovine, respectively. Reactivity has been confirmed on western blots with human HeLa and WI-38 cell lysates as well as rat KNRK and mouse NIH 3T3 cell lysates, and identifies the target band at~21 kDa. The reactivity has been also confirmed with rat KNRK cells using immunoprecipitation and with HeLa cells by immunofluorescence. Based on amino acid sequence homology, reactivity with bovine is also expected.

靶标信息

K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

KRAS-specific antibody binds to KRAS protein inside colorectal adenocarcinoma cells and inhibits its localization to the plasma membrane.

Frontiers in oncology

Lam KK,Low YS,Lo M,Wong M,Leong Tang C,Tan E,Chok AY,Seow-En I,Wong SH,Cheah PY

415700 was used in Sample prep to demonstrate that internalized anti-KRAS antibody inhibits KRAS function in tumor.

Sat Apr 15 00:00:00 EDT 2023

CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities.

Nature

Han K,Pierce SE,Li A,Spees K,Anderson GR,Seoane JA,Lo YH,Dubreuil M,Olivas M,Kamber RA,Wainberg M,Kostyrko K,Kelly MR,Yousefi M,Simpkins SW,Yao D,Lee K,Kuo CJ,Jackson PK,Sweet-Cordero A,Kundaje A,Gentles AJ,Curtis C,Winslow MM,Bassik MC

415700 was used in Western Blotting to reveal key differences between 2D and 3D cancer models, and establish a generalizable strategy for performing CRISPR screens in spheroids to reveal cancer vulnerabilities.

Wed Apr 01 00:00:00 EDT 2020

CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities.

Nature

Wed Apr 01 00:00:00 EDT 2020

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Cell chemical biology

Röth S,Macartney TJ,Konopacka A,Chan KH,Zhou H,Queisser MA,Sapkota GP

Published figure using K-Ras monoclonal antibody (Product # 415700) in Western Blot

Thu Sep 17 00:00:00 EDT 2020

Integrated analysis of patient samples identifies biomarkers for venetoclax efficacy and combination strategies in acute myeloid leukemia.

Nature cancer

Zhang H,Nakauchi Y,Köhnke T,Stafford M,Bottomly D,Thomas R,Wilmot B,McWeeney SK,Majeti R,Tyner JW

415700 was used in Western Blot to show that Venetoclax in combination with an MCL1 inhibitor AZD5991 induced synthetic lethality and circumvented venetoclax resistance.

Sat Aug 01 00:00:00 EDT 2020

A Novel KRAS Antibody Highlights a Regulation Mechanism of Post-Translational Modifications of KRAS during Tumorigenesis.

International journal of molecular sciences

Assi M,Pirlot B,Stroobant V,Thissen JP,Jacquemin P

415700 was used in Western Blotting to find that KRAS prenylation was increased ex vivo and in vivo in a model of KRASG12D-driven tumorigenesis, which was concomitant with an induction of expression of essential KRAS prenylation enzymes.

Wed Sep 02 00:00:00 EDT 2020

Targeting wild-type KRAS-amplified gastroesophageal cancer through combined MEK and SHP2 inhibition.

Nature medicine

Wong GS,Zhou J,Liu JB,Wu Z,Xu X,Li T,Xu D,Schumacher SE,Puschhof J,McFarland J,Zou C,Dulak A,Henderson L,Xu P,O'Day E,Rendak R,Liao WL,Cecchi F,Hembrough T,Schwartz S,Szeto C,Rustgi AK,Wong KK,Diehl JA,Jensen K,Graziano F,Ruzzo A,Fereshetian S,Mertins P,Carr SA,Beroukhim R,Nakamura K,Oki E,Watanabe M,Baba H,Imamura Y,Catenacci D,Bass AJ

415700 was used in Immunohistochemistry to examine focal high-level amplification of the KRAS gene in the absence of coding mutations, as a secondary means of KRAS activation in cancer.

Sun Jul 01 00:00:00 EDT 2018

The proto-oncogene KRAS is targeted by miR-200c.

Oncotarget

Kopp F,Wagner E,Roidl A

415700 was used in western blot to demonstrate that miR-200c regulates KRAS.

Wed Jan 15 00:00:00 EST 2014

生物信息学

蛋白别名: c-K-ras; c-K-ras2 protein; c-Ki-ras; c-Kirsten-ras protein; c-Kirsten-ras proto-oncogene; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; GTPase KRas; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral oncogene homologue 2 (active); oncogene KRAS2; p21 protein; PR310 c-K-ras oncogene; RASK; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

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基因别名: AI929937; C-K-RAS; c-Ki-ras; CFC2; K-ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; Kras-2; KRAS1; KRAS2; NS; NS3; p21; p21B; RALD; ras; RASK2

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UniProt ID: (Human) P01116, (Rat) P08644, (Mouse) P32883

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Entrez Gene ID: (Human) 3845, (Rat) 24525, (Mouse) 16653

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功能

GTPase activity protein binding GTP binding GMP binding GDP binding LRR domain binding protein complex binding nucleotide binding small GTPase

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