Invitrogen

KDEL Monoclonal Antibody (2C1), FITC

KDEL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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KDEL Antibody (MA5-45870) in IHC (P)

Immunohistochemistry analysis using kidney. Fixation involved formalin fixed, paraffin embedded treatment. Samples were incubated with KDEL monoclonal antibody (Product # MA5-45870) at 1:25 for 1 hour at RT, followed by Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain used was Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phallo... View More

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产品信息

MA5-45870

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:25

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2C1

抗原

KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)

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偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

内含物

no preservative

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2932324

产品详细信息

A 1:1,000 dilution of MA5-45870 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects KDEL tagged proteins.

靶标信息

The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 78 kDa glucose-regulated protein; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; ER lumen protein-retaining receptor 1; ERp99; glucose regulated protein, 78 kDa; glucose-regulated protein, 78kDa; gp96 homolog; GRP 78; GRP-78; GRP-94; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family A member 5; Heat shock protein family C member 4; HSP70 family protein 5; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Immunoglobulin heavy chain-binding protein; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; Polymorphic tumor rejection antigen 1; Putative MAPK-activating protein PM23; RP23-446N16.1; Steroidogenesis-activator polypeptide; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; XAP-1 antigen

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基因别名: 8030486F04Rik; AL022860; AU019543; AW215843; baffled; BIP; D2Wsu141e; D2Wsu17e; ECGP; endoplasmin; ERD2; ERD2.1; ERp99; GP96; GRP78; GRP94; HDEL; HEL-S-125m; HEL-S-89n; HEL35; Hsce70; HSP90B1; HSPA5; HSPC4; KDELR1; mBiP; MIF2; PM23; SEZ-7; Sez7; TA-3; Targ2; Tra-1; TRA1