Invitrogen

KDEL Monoclonal Antibody (2D6)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

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KDEL Antibody (MA5-27581) in WB

Western blot was performed using KDEL Monoclonal Antibody (2D6) (Product # MA5-27581) and 94, 78, 55 kDa bands corresponding to GRP94, GRP78 and P4HB (PDI) that all share the C-terminal KDEL sequence were observed and upregulated upon thapsigargin and tunicamycin treatments. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MCF7 treated with thapsigargin (Lane 2), HeLa (Lane 3), HeLa treated with thapsigargin (Lane 4), HCT 116 (Lane 5), HCT 116 treated with tunicamycin (Lane 6),C2C12 (Lane 7), PC-3 (Lane 8), Mouse Liver (Lane 9), Rat Te... View More

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KDEL Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HCT 116 cells were transfected with GRP78, GRP94, P4HB (PDI) siRNA and decrease in signal intensity was observed in Western Blot application using KDEL Monoclonal Antibody (2D6) (Product # MA5-27581). {KD}

产品信息

MA5-27581

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Chemical

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2D6

抗原

KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2735251

产品详细信息

A 1:1,000 dilution of MA5-27581 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects KDEL tagged proteins.

靶标信息

The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Secretion of a low-molecular-weight species of endogenous GRP94 devoid of the KDEL motif during endoplasmic reticulum stress in Chinese hamster ovary cells.

Traffic (Copenhagen, Denmark)

Samy A,Yamano-Adachi N,Koga Y,Omasa T

MA5-27581 was used in Immunocytochemistry-immunofluorescence to show that endogenous GRP94 is naturally secreted into the medium in a truncated form that lacks the KDEL motif in Chinese hamster ovary cells.

Wed Dec 01 00:00:00 EST 2021

生物信息学

蛋白别名: 78 kDa glucose-regulated protein; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; ER lumen protein-retaining receptor 1; ERp99; glucose regulated protein, 78 kDa; glucose-regulated protein, 78kDa; gp96 homolog; GRP 78; GRP-78; GRP-94; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family A member 5; Heat shock protein family C member 4; HSP70 family protein 5; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Immunoglobulin heavy chain-binding protein; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; Polymorphic tumor rejection antigen 1; Putative MAPK-activating protein PM23; RP23-446N16.1; Steroidogenesis-activator polypeptide; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; XAP-1 antigen

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基因别名: 8030486F04Rik; AL022860; AU019543; AW215843; baffled; BIP; D2Wsu141e; D2Wsu17e; ECGP; endoplasmin; ERD2; ERD2.1; ERp99; GP96; GRP78; GRP94; HDEL; HEL-S-125m; HEL-S-89n; HEL35; Hsce70; HSP90B1; HSPA5; HSPC4; KDELR1; mBiP; MIF2; PM23; SEZ-7; Sez7; TA-3; Targ2; Tra-1; TRA1