Invitrogen

KEAP1 Polyclonal Antibody

KEAP1 Antibody in Immunocytochemistry (ICC/IF)

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KEAP1 Antibody (PA5-142760) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of KEAP1 in HeLa cells using KEAP1 Polyclonal Antibody (Product # PA5-142760). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (5 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

KEAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-142760

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

3-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide with sequence C-EVTPSQHGNRTFS.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2933406

产品详细信息

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.

靶标信息

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。