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KIAA0391 Antibody (PA5-96243) in WB
Western blot analysis of KIAA0391 in extracts of various cell lines. Samples were incubated with KIAA0391 Polyclonal antibody (Product # PA5-96243) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
产品信息
PA5-96243
产品规格
种属反应
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 314-583 of human KIAA0391 (NP_0554872)
偶联物
形式
Liquid
浓度
0.59 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808045
产品详细信息
Immunogen sequence: YPGESFAHSI KTWFESVPGK QWKGQFTTVR KSGQCSGCGK TIESIQLSPE EYECLKGKIM RDVIDGGDQY RKTTPQELKR FENFIKSRPP FDVVIDGLNV AKMFPKVRES QLLLNVVSQL AKRNLRLLVL GRKHMLRRSS QWSRDEMEEV QKQASCFFAD DISEDDPFLL YATLHSGNHC RFITRDLMRD HKACLPDAKT QRLFFKWQQG HQLAIVNRFP GSKLTFQRIL SYDTVVQTTG DSWHIPYDED LVERCSCEVP TKWLCLHQKT; Positive Samples: Rat testis, Rat brain; Cellular Location: Mitochondrion
靶标信息
KIAA0391, also known as MRPP3 (mitochondrial ribonuclease P protein 3), is a 583 amino acid protein that is located in the mitochondria. KIAA0391 functions in tRNA maturation and is a component of mitochondrial ribonuclease P which cleaves tRNA at the 5'-end. Mitochondrial ribonuclease P is an enzyme composed of RG9MTD1, MRPP22 and KIAA0391 subunits. Due to alternative splicing events, KIAA0391 exists as four isoforms. The gene encoding KIAA0391 maps to human chromosome 14, which contains about 700 genes and makes up about 3. 5% of human cellular DNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Mitochondrial ribonuclease P catalytic subunit; Mitochondrial ribonuclease P protein 3; Mitochondrial RNase P protein 3
基因别名: Mrpp3; Prorp
UniProt ID: (Rat) B5DF07
Entrez Gene ID: (Rat) 299050
