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KIF23 Antibody (PA5-100863) in ICC/IF
Immunocytochemistry analysis of KIF23 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with KIF23 polyclonal antibody (Product # PA5-100863) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-100863
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human KIF23(Accession Q02241), corresponding to amino acid residues V432-S482.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2850361
产品详细信息
Antibody detects endogenous levels of total KIF23.
靶标信息
The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in two transcript variants encoding two different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: kinesin-like 5 (mitotic kinesin-like protein 1); Kinesin-like protein 5; Kinesin-like protein KIF23; Mitotic kinesin-like protein 1
基因别名: 3110001D19Rik; C87313; CHO1; KIF23; KNSL5; MKLP-1; MKLP1
UniProt ID: (Human) Q02241, (Mouse) E9Q5G3
Entrez Gene ID: (Human) 9493, (Mouse) 71819
mitotic spindle elongation
mitotic cytokinesis
cytokinesis
actomyosin contractile ring assembly
retrograde vesicle-mediated transport, Golgi to ER
microtubule-based movement
antigen processing and presentation of exogenous peptide antigen via MHC class II
positive regulation of cytokinesis
mitotic spindle midzone assembly
plus-end-directed vesicle transport along microtubule
cell cycle
mitotic nuclear division
cell division
